来自S-羧甲基角蛋白-A的α-螺旋片段的氨基酸序列。II型片段的完整序列。
Amino acid sequences of alpha-helical segments from S-carboxymethylkerateine-A. Complete sequence of a type-II segment.
作者信息
Crewther W G, Inglis A S, McKern N M
出版信息
Biochem J. 1978 Aug 1;173(2):365-71. doi: 10.1042/bj1730365.
Abstract
- The helical fragments obtained by partial chymotryptic digestion of S-carboxymethylkeratine-A, the low-sulphur fraction from wool, were fractionated into type-I and type-II helical segments in aqueous urea under conditions limiting carbamoylation. 2. The amino acid sequence of a 109-residue type-II segment was completed by using the sequenator. 3. When the data were incorporated into a helical model of 3.6 residues per turn the hydrophobic residues generated a band aligned at a slight angle to the helical axis. This result is in accord with the postulated coiled-coil structure of the crystalline regions of alpha-keratin.
摘要
- 通过对羊毛中的低硫组分S-羧甲基角蛋白-A进行部分胰凝乳蛋白酶消化得到的螺旋片段，在限制氨甲酰化的条件下，于尿素水溶液中被分离为I型和II型螺旋片段。2. 使用序列分析仪完成了一个109个残基的II型片段的氨基酸序列测定。3. 当将这些数据纳入每圈3.6个残基的螺旋模型时，疏水残基形成了一条与螺旋轴成小角度排列的带。这一结果与α-角蛋白晶体区域的假定卷曲螺旋结构一致。
相似文献
1
Amino acid sequences of alpha-helical segments from S-carboxymethylkerateine-A. Complete sequence of a type-II segment.来自S-羧甲基角蛋白-A的α-螺旋片段的氨基酸序列。II型片段的完整序列。
Biochem J. 1978 Aug 1;173(2):365-71. doi: 10.1042/bj1730365.
2
Amino acid sequences of alpha-helical segments from S-carboxymethylkerateine-A. Tryptic and chymotryptic peptides from a type-II segment.来自S-羧甲基角蛋白-A的α-螺旋片段的氨基酸序列。来自II型片段的胰蛋白酶和胰凝乳蛋白酶肽段。
Biochem J. 1978 Aug 1;173(2):353-63. doi: 10.1042/bj1730353.
3
Amino acid sequences of alpha-helical segments from S-carbosymethylkerateine-A. Complete sequence of a type-I segment.来自S-羧甲基角蛋白A的α-螺旋片段的氨基酸序列。I型片段的完整序列。
Biochem J. 1978 Aug 1;173(2):373-85. doi: 10.1042/bj1730373.
4
Amino acid sequences of alpha-helical segments from S-carboxymethylkerateine-A. Statistical analysis.来自S-羧甲基角蛋白A的α-螺旋片段的氨基酸序列。统计分析。
Biochem J. 1978 Aug 1;173(2):387-91. doi: 10.1042/bj1730387.
5
The primary structure of component 8c-1, a subunit protein of intermediate filaments in wool keratin. Relationships with proteins from other intermediate filaments.羊毛角蛋白中间丝亚基蛋白8c-1的一级结构。与其他中间丝蛋白的关系。
Biochem J. 1986 Jun 15;236(3):695-703. doi: 10.1042/bj2360695.
6
Amino acid sequences of peptides from a chymotryptic digest of a urea-soluble protein fraction (U.S.3) from oxidized wool.来自氧化羊毛的尿素可溶蛋白组分（U.S.3）的胰凝乳蛋白酶消化产物中肽段的氨基酸序列。
Biochem J. 1969 Nov;115(2):323-34. doi: 10.1042/bj1150323.
7
Aggregation of wool keratin intermediate filament proteins.羊毛角蛋白中间丝蛋白的聚集
Int J Biol Macromol. 1989 Oct;11(5):278-84. doi: 10.1016/0141-8130(89)90020-2.
8
The amino acid sequence of protein SCMK-B2C from the high-sulphur fraction of wool keratin.羊毛角蛋白高硫组分中蛋白质SCMK-B2C的氨基酸序列。
Biochem J. 1972 Aug;128(5):1229-39. doi: 10.1042/bj1281229.
9
The amino acid sequence of protein SCMK-B2A from the high-sulphur fraction of wool keratin.羊毛角蛋白高硫组分中蛋白质SCMK-B2A的氨基酸序列。
Biochem J. 1972 Dec;130(3):833-45. doi: 10.1042/bj1300833.
10
Evidence of homology in a high-sulphur protein fraction (SCMK-B2) of wool and hair alpha-keratins.羊毛和毛发α-角蛋白的高硫蛋白质组分（SCMK-B2）中同源性的证据。
Biochem J. 1968 Nov;110(2):193-200. doi: 10.1042/bj1100193.
引用本文的文献
1
Structures of the ß-Keratin Filaments and Keratin Intermediate Filaments in the Epidermal Appendages of Birds and Reptiles (Sauropsids).鸟类和爬行类（蜥臀目）表皮附属物中的β-角蛋白丝和角蛋白中间丝的结构。
Genes (Basel). 2021 Apr 17;12(4):591. doi: 10.3390/genes12040591.
2
Functional domains of the InsA protein of IS2.IS2的InsA蛋白的功能结构域。
J Bacteriol. 1997 Oct;179(20):6238-43. doi: 10.1128/jb.179.20.6238-6243.1997.
3
Comparison of the proteins of two immunologically distinct intermediate-sized filaments by amino acid sequence analysis: desmin and vimentin.通过氨基酸序列分析比较两种免疫特性不同的中间丝蛋白：结蛋白和波形蛋白。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4120-3. doi: 10.1073/pnas.78.7.4120.
4
Structural studies on the microfibrillar proteins of wool. Interaction between alpha-helical segments and reassembly of a four-chain structure.羊毛微原纤维蛋白的结构研究。α-螺旋片段之间的相互作用及四链结构的重新组装。
Biochem J. 1983 Mar 1;209(3):587-95. doi: 10.1042/bj2090587.
5
Repeats of base oligomers as the primordial coding sequences of the primeval earth and their vestiges in modern genes.作为原始地球原始编码序列的碱基寡聚物重复序列及其在现代基因中的遗迹。
J Mol Evol. 1984;20(3-4):313-21. doi: 10.1007/BF02104737.
6
Reconstitution of cytokeratin filaments in vitro: further evidence for the role of nonhelical peptides in filament assembly.细胞角蛋白丝在体外的重组：非螺旋肽在丝组装中作用的进一步证据。
J Cell Biol. 1984 Nov;99(5):1590-7. doi: 10.1083/jcb.99.5.1590.
7
The structural relation between intermediate filament proteins in living cells and the alpha-keratins of sheep wool.活细胞中中间丝蛋白与羊毛α-角蛋白之间的结构关系。
EMBO J. 1982;1(10):1155-60. doi: 10.1002/j.1460-2075.1982.tb00006.x.
8
Intermediate filaments of baby hamster kidney (BHK-21) cells and bovine epidermal keratinocytes have similar ultrastructures and subunit domain structures.幼仓鼠肾（BHK - 21）细胞和牛表皮角质形成细胞的中间丝具有相似的超微结构和亚基结构域结构。
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4534-8. doi: 10.1073/pnas.77.8.4534.
9
Molecular cloning and primary structure of human glial fibrillary acidic protein.人胶质纤维酸性蛋白的分子克隆与一级结构
Proc Natl Acad Sci U S A. 1989 Jul;86(13):5178-82. doi: 10.1073/pnas.86.13.5178.
10
Concerted gene duplications in the two keratin gene families.
J Mol Evol. 1988;27(3):203-11. doi: 10.1007/BF02100075.
本文引用的文献
1
The isomeric transformation of urea into ammonium cyanate in aqueous solutions.尿素在水溶液中异构转化为氰酸铵。
Biochem J. 1948;42(4):628-32.
2
A protein sequenator.蛋白质测序仪。
Eur J Biochem. 1967 Mar;1(1):80-91. doi: 10.1007/978-3-662-25813-2_14.
3
X-ray diffraction and infrared studies of an -helical fragment from -keratin.
J Mol Biol. 1973 Jan 10;73(2):275-8. doi: 10.1016/0022-2836(73)90329-x.
4
Isolation of components from the low-sulphur proteins of wool by fractional precipitation.
Prep Biochem. 1974;4(3):203-26. doi: 10.1080/00327487408068199.
5
Automatic procedures for determining amino acid sequences of peptides.
Anal Biochem. 1975 Oct;68(2):572-85. doi: 10.1016/0003-2697(75)90653-3.
6
Fourteen actin-binding sites on tropomyosin?
Nature. 1975 Sep 25;257(5524):331-3. doi: 10.1038/257331a0.
7
8
Amino acid sequences of alpha-helical segments from S-carboxymethylkerateine-A. Tryptic and chymotryptic peptides from a type-II segment.来自S-羧甲基角蛋白-A的α-螺旋片段的氨基酸序列。来自II型片段的胰蛋白酶和胰凝乳蛋白酶肽段。
Biochem J. 1978 Aug 1;173(2):353-63. doi: 10.1042/bj1730353.
Zotero 插件