来自氧化羊毛的尿素可溶蛋白组分（U.S.3）的胰凝乳蛋白酶消化产物中肽段的氨基酸序列。
Amino acid sequences of peptides from a chymotryptic digest of a urea-soluble protein fraction (U.S.3) from oxidized wool.
作者信息
Corfield M C, Fletcher J C
出版信息
Biochem J. 1969 Nov;115(2):323-34. doi: 10.1042/bj1150323.
Abstract
- A chymotryptic digest of the protein fraction U.S.3. from oxidized wool was separated into 51 peptide fractions by chromatography on a column of cation-exchange resin. 2. The less acidic fractions were separated into their component peptides by a combination of cation-exchange-resin chromatography, paper chromatography and paper electrophoresis. 3. The amino acid sequences of 34 of these peptides were elucidated, and those of 14 others partially determined. 4. Overlaps between the tryptic and chymotryptic peptides from fraction U.S.3 have enabled ten extended amino acid sequences to be deduced, the longest containing 20 amino acid residues. 5. The relevance of the results to the structures of the helical and non-helical regions of wool is discussed.
摘要
- 对氧化羊毛的蛋白质组分U.S.3进行胰凝乳蛋白酶消化，通过阳离子交换树脂柱色谱法将其分离成51个肽段。2. 酸性较弱的组分通过阳离子交换树脂色谱法、纸色谱法和纸电泳法相结合的方式分离成其组成肽段。3. 阐明了其中34个肽段的氨基酸序列，部分确定了另外14个肽段的氨基酸序列。4. 来自组分U.S.3的胰蛋白酶肽段和胰凝乳蛋白酶肽段之间的重叠使得能够推导十个延伸的氨基酸序列，最长的包含20个氨基酸残基。5. 讨论了这些结果与羊毛螺旋区和非螺旋区结构的相关性。
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本文引用的文献
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Amino acid sequences of peptides from a tryptic digest of a urea-soluble protein fraction (U.S.3) from oxidized wool.氧化羊毛尿素可溶蛋白部分（U.S.3）经胰蛋白酶消化后得到的肽的氨基酸序列。
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