苏云金芽孢杆菌柏林亚种杀虫外毒素的纯化及某些特性
A purification and some properties of an insecticidal exotoxin from Bacillus thuringiensis Berliner.
作者信息
Bond R P, Boyce C B, French S J
出版信息
Biochem J. 1969 Sep;114(3):477-88. doi: 10.1042/bj1140477.
Abstract
An insecticidal exotoxin from Bacillus thuringiensis var. thuringiensis (Berliner) has been purified. The efficiency of each stage of the purification has been ascertained and the yield of toxic material estimated by means of a quantitative bioassay. It is shown that the exotoxin is an adenine derivative substituted at position 9 and having a molecular weight of approximately 825. It can be dephosphorylated enzymically or chemically under conditions that define the exotoxin as a phosphomonoester. This results in loss of toxicity, both to insects and to mice. Spectroscopic and kinetic data are presented which suggest that a beta-ribofuranosyl moiety may be attached to the adenine. Glucose and allomucic acid have been positively identified as hydrolysis fragments from the exotoxin. These results are discussed and compared with the results of others on similar (or possibly identical) compounds.
摘要
苏云金芽孢杆菌苏云金变种（Berliner）的一种杀虫外毒素已被纯化。已确定了纯化各阶段的效率，并通过定量生物测定法估计了有毒物质的产量。结果表明，该外毒素是一种在9位被取代的腺嘌呤衍生物，分子量约为825。在将外毒素定义为磷酸单酯的条件下，它可通过酶法或化学法进行去磷酸化。这会导致对昆虫和小鼠的毒性丧失。给出了光谱和动力学数据，这些数据表明β-呋喃核糖基部分可能连接在腺嘌呤上。已明确鉴定出葡萄糖和别粘酸是外毒素的水解片段。对这些结果进行了讨论，并与其他关于类似（或可能相同）化合物的结果进行了比较。
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