苏云金芽孢杆菌产生的一种外毒素对哺乳动物核糖核酸聚合酶的差异性抑制作用。完整细胞核中核质核糖核酸聚合酶活性的直接观察。
Differential inhibition of mammalian ribonucleic acid polymerases by an exotoxin from Bacillus thuringiensis. The direct observation of nucleoplasmic ribonucleic acid polymerase activity in intact nuclei.
作者信息
Beebee T, Korner A, Bond R P
出版信息
Biochem J. 1972 May;127(4):619-34. doi: 10.1042/bj1270619.
Abstract
The effects of the exotoxin from Bacillus thuringiensis on DNA-dependent RNA polymerases from rat liver were examined. The exotoxin inhibits all RNA polymerase activity at both low and high ionic strength in intact nuclei, and soluble enzymes are similarly affected. This inhibition is relieved by ATP. Dephosphorylated exotoxin did not inhibit the soluble enzymes. Nucleolar and nucleoplasmic RNA polymerases respond to different concentration ranges of exotoxin, and the compound can be used in intact nuclei to isolate the nucleoplasmic activity.
摘要
研究了苏云金芽孢杆菌外毒素对大鼠肝脏中依赖DNA的RNA聚合酶的影响。该外毒素在完整细胞核中，无论在低离子强度还是高离子强度下，均能抑制所有RNA聚合酶活性，对可溶性酶也有类似影响。ATP可解除这种抑制作用。脱磷酸化的外毒素不抑制可溶性酶。核仁RNA聚合酶和核质RNA聚合酶对外毒素的浓度范围反应不同，该化合物可用于完整细胞核中以分离核质活性。
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