由长链阴离子激活产生的磷脂酶D最佳pH值的变化。
A shift in the optimum pH of pospholipase D produced by activating long-chain anions.
作者信息
Quarles R H, Dawson R M
出版信息
Biochem J. 1969 May;112(5):795-9. doi: 10.1042/bj1120795.
Abstract
- The activity of phospholipase D (phosphatidylcholine phosphatidohydrolase, EC 3.1.4.4) towards ultrasonically treated phosphatidylcholine or large phosphatidylcholine particles activated with ether was maximal near pH5, and there was little activity above pH6. 2. When the enzyme was activated by the addition of phosphatidic acid to large phosphatidylcholine particles the pH optimum was shifted to pH6.5 irrespective of the amount of activator added. 3. When the enzyme was activated with low concentrations of dodecyl sulphate the pH optimum was 5.5 with little activity above pH6. With higher concentrations of dodecyl sulphate the pH-activity profile was shifted upwards towards a pH optimum of 6.5-6.6, the magnitude of the shift depending on the extent of the hydrolysis. 4. The shifts in the pH-activity profiles cannot be correlated with changes in the ;surface pH' of the substrate particles calculated from the measurement of their zeta-potentials (electrophoretic mobilities).
摘要
- 磷脂酶D（磷脂酰胆碱磷脂水解酶，EC 3.1.4.4）对经超声处理的磷脂酰胆碱或用乙醚活化的大磷脂酰胆碱颗粒的活性在pH5附近最大，在pH6以上活性很低。2. 当通过向大磷脂酰胆碱颗粒中添加磷脂酸来激活该酶时，无论添加的激活剂数量如何，最适pH都移至pH6.5。3. 当用低浓度的十二烷基硫酸盐激活该酶时，最适pH为5.5，在pH6以上活性很低。使用较高浓度的十二烷基硫酸盐时，pH-活性曲线向上移动，最适pH为6.5 - 6.6，移动幅度取决于水解程度。4. pH-活性曲线的移动与根据底物颗粒的ζ电位（电泳迁移率）测量计算出的底物颗粒“表面pH”的变化无关。
相似文献
1
A shift in the optimum pH of pospholipase D produced by activating long-chain anions.由长链阴离子激活产生的磷脂酶D最佳pH值的变化。
Biochem J. 1969 May;112(5):795-9. doi: 10.1042/bj1120795.
2
Some properties of purified phospholipase D and especially the effect of amphipathic substances.纯化磷脂酶D的一些特性，尤其是两亲性物质的作用。
Biochem J. 1967 Jan;102(1):76-86. doi: 10.1042/bj1020076.
3
The hydrolysis of monolayers of phosphatidyl(Me-14C)choline by phospholipase D.磷脂酶D对磷脂酰（甲基 - 14C）胆碱单层膜的水解作用。
Biochem J. 1969 Jul;113(4):697-705. doi: 10.1042/bj1130697.
4
Phospholipase D (phosphatidylcholine phosphatidohydrolase, EC 3.1.4.4) of Cicer arietinum.鹰嘴豆的磷脂酶D（磷脂酰胆碱磷脂水解酶，EC 3.1.4.4）
Indian J Biochem. 1969 Dec;6(4):228-30.
5
Studies of dialkyl ether phospholipids. II. Requirement for a liquid-crystalline substrate for hydrolysis by cabbage leaf phospholipase D.二烷基醚磷脂的研究。II. 白菜叶磷脂酶D水解所需的液晶底物
Can J Biochem. 1971 Dec;49(12):1362-75. doi: 10.1139/o71-197.
6
[Phospholipase D - a review (author's transl)].磷脂酶D——综述（作者译）
Z Lebensm Unters Forsch. 1975 Nov 24;159(4):225-33. doi: 10.1007/BF01460064.
7
Isolation and characterization of phospholipase D from fababeans.
Lipids. 1979 Nov;14(11):913-7. doi: 10.1007/BF02533505.
8
The phospholipid-dependence of uridine diphosphate glucuronyltransferase. Reactivation of phospholipase A-inactivated enzyme by phospholipids and detergents.尿苷二磷酸葡糖醛酸基转移酶的磷脂依赖性。磷脂酶A失活的酶被磷脂和去污剂重新激活。
Biochem J. 1971 Aug;123(5):875-82. doi: 10.1042/bj1230875.
9
Properties of the phospholipase D from peanut seeds.
Biochim Biophys Acta. 1970 Jul 14;210(2):276-86. doi: 10.1016/0005-2760(70)90172-4.
10
THE ENZYMIC HYDROLYSIS OF ULTRASONICALLY IRRADIATED LECITHIN BY PHOSPHOLIPASE A.磷脂酶A对超声辐照卵磷脂的酶促水解作用
Biochim Biophys Acta. 1963 Dec 27;70:697-9. doi: 10.1016/0006-3002(63)90816-3.
引用本文的文献
1
Possible involvement of a glycerophosphate compound in auxin induced growth.可能涉及到甘油磷酸盐化合物在生长素诱导生长中的作用。
Planta. 1971 Mar;99(1):56-62. doi: 10.1007/BF00392120.
2
Freezing Injury and Phospholipid Degradation in Vivo in Woody Plant Cells: II. Regulatory Effects of Divalent Cations on Activity of Membrane-bound Phospholipase D.木本植物细胞中的冻害与磷脂降解：二、二价阳离子对膜结合型磷脂酶 D 活性的调节作用。
Plant Physiol. 1979 Aug;64(2):247-51. doi: 10.1104/pp.64.2.247.
3
Phosphatidylinositol phosphodiesterase in higher plants.高等植物中的磷脂酰肌醇磷酸二酯酶。
Biochem J. 1980 Oct 15;192(1):279-83. doi: 10.1042/bj1920279.
4
The hydrolysis of monolayers of phosphatidyl(Me-14C)choline by phospholipase D.磷脂酶D对磷脂酰（甲基 - 14C）胆碱单层膜的水解作用。
Biochem J. 1969 Jul;113(4):697-705. doi: 10.1042/bj1130697.
5
The enzymic deacylation of phospholipids and galactolipids in plants. Purification and properties of a lipolytic acyl-hydrolase from potato tubers.植物中磷脂和半乳糖脂的酶促脱酰作用。马铃薯块茎中一种脂解酰基水解酶的纯化及性质
Biochem J. 1971 Feb;121(3):379-90. doi: 10.1042/bj1210379.
6
Lipoxygenase from potato tubers. Partial purification and properties of an enzyme that specifically oxygenates the 9-position of linoleic acid.来自马铃薯块茎的脂氧合酶。一种特异性氧化亚油酸9位的酶的部分纯化及性质
Biochem J. 1971 Sep;124(2):431-8. doi: 10.1042/bj1240431.
7
Cardiolipin-specific phospholipase D activity in Haemophilus parainfluenzae.副流感嗜血杆菌中的心磷脂特异性磷脂酶D活性
J Bacteriol. 1970 Jul;103(1):111-5. doi: 10.1128/jb.103.1.111-115.1970.
8
Lysosomes and the connective tissue diseases.溶酶体与结缔组织疾病
J Clin Pathol Suppl (R Coll Pathol). 1978;12:105-16.
9
The effect of phospholipase D on the function of fragmented sarcoplasmic reticulum.
Lipids. 1978 Apr;13(4):264-6. doi: 10.1007/BF02533667.
本文引用的文献
1
A WATER-INSOLUBLE POLYANIONIC DERIVATIVE OF TRYPSIN. II. EFFECT OF THE POLYELECTROLYTE CARRIER ON THE KINETIC BEHAVIOR OF THE BOUND TRYPSIN.一种胰蛋白酶的水不溶性聚阴离子衍生物。II. 聚电解质载体对结合型胰蛋白酶动力学行为的影响。
Biochemistry. 1964 Dec;3:1913-9. doi: 10.1021/bi00900a022.
2
ON THE MECHANISM OF ACTION OF PHOSPHOLIPASE A.关于磷脂酶A的作用机制
Biochem J. 1963 Sep;88(3):414-23. doi: 10.1042/bj0880414.
3
An apparatus for microelectrophoresis of small particles.一种用于小颗粒微电泳的装置。
Nature. 1958 Sep 6;182(4636):642-4. doi: 10.1038/182642a0.
4
The structure of the naturally occurring phosphoglycerides. 4. Action of cabbage-leaf phospholipase D on ovolecithin and related substances.天然存在的磷酸甘油酯的结构。4. 卷心菜叶磷脂酶D对卵黄卵磷脂及相关物质的作用。
Biochem J. 1958 Jul;69(3):458-66. doi: 10.1042/bj0690458.
5
Influence of pH on the activity of chymotrypsin at a solid-liquid interface.pH对固液界面处胰凝乳蛋白酶活性的影响。
Arch Biochem Biophys. 1957 May;68(1):157-60. doi: 10.1016/0003-9861(57)90336-3.
6
Lecithinase systems in sugar beet, spinach, cabbage, and carrot.甜菜、菠菜、卷心菜和胡萝卜中的卵磷脂酶系统。
Can J Biochem Physiol. 1954 Sep;32(5):571-83.
7
The phosphatides of the latex of Hevea brasiliensis. 1. Biochemical changes.巴西橡胶树胶乳中的磷脂。1. 生化变化。
Biochem J. 1954 Feb;56(2):240-50. doi: 10.1042/bj0560240.
8
Some properties of purified phospholipase D and especially the effect of amphipathic substances.纯化磷脂酶D的一些特性，尤其是两亲性物质的作用。
Biochem J. 1967 Jan;102(1):76-86. doi: 10.1042/bj1020076.
9
The preparation and properties of ficin chemically attached to carboxymethylcellulose.化学连接到羧甲基纤维素上的无花果蛋白酶的制备及其性质
Biochem J. 1966 Feb;98(2):420-5. doi: 10.1042/bj0980420.
Zotero 插件