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Biochem J. 1969 May;112(5):795-9. doi: 10.1042/bj1120795.
2
Some properties of purified phospholipase D and especially the effect of amphipathic substances.纯化磷脂酶D的一些特性，尤其是两亲性物质的作用。
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3
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4
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Studies of dialkyl ether phospholipids. II. Requirement for a liquid-crystalline substrate for hydrolysis by cabbage leaf phospholipase D.二烷基醚磷脂的研究。II. 白菜叶磷脂酶D水解所需的液晶底物
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8
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本文引用的文献

1
A WATER-INSOLUBLE POLYANIONIC DERIVATIVE OF TRYPSIN. II. EFFECT OF THE POLYELECTROLYTE CARRIER ON THE KINETIC BEHAVIOR OF THE BOUND TRYPSIN.一种胰蛋白酶的水不溶性聚阴离子衍生物。II. 聚电解质载体对结合型胰蛋白酶动力学行为的影响。
Biochemistry. 1964 Dec;3:1913-9. doi: 10.1021/bi00900a022.
2
ON THE MECHANISM OF ACTION OF PHOSPHOLIPASE A.关于磷脂酶A的作用机制
Biochem J. 1963 Sep;88(3):414-23. doi: 10.1042/bj0880414.
3
An apparatus for microelectrophoresis of small particles.一种用于小颗粒微电泳的装置。
Nature. 1958 Sep 6;182(4636):642-4. doi: 10.1038/182642a0.
4
The structure of the naturally occurring phosphoglycerides. 4. Action of cabbage-leaf phospholipase D on ovolecithin and related substances.天然存在的磷酸甘油酯的结构。4. 卷心菜叶磷脂酶D对卵黄卵磷脂及相关物质的作用。
Biochem J. 1958 Jul;69(3):458-66. doi: 10.1042/bj0690458.
5
Influence of pH on the activity of chymotrypsin at a solid-liquid interface.pH对固液界面处胰凝乳蛋白酶活性的影响。
Arch Biochem Biophys. 1957 May;68(1):157-60. doi: 10.1016/0003-9861(57)90336-3.
6
Lecithinase systems in sugar beet, spinach, cabbage, and carrot.甜菜、菠菜、卷心菜和胡萝卜中的卵磷脂酶系统。
Can J Biochem Physiol. 1954 Sep;32(5):571-83.
7
The phosphatides of the latex of Hevea brasiliensis. 1. Biochemical changes.巴西橡胶树胶乳中的磷脂。1. 生化变化。
Biochem J. 1954 Feb;56(2):240-50. doi: 10.1042/bj0560240.
8
Some properties of purified phospholipase D and especially the effect of amphipathic substances.纯化磷脂酶D的一些特性，尤其是两亲性物质的作用。
Biochem J. 1967 Jan;102(1):76-86. doi: 10.1042/bj1020076.
9
The preparation and properties of ficin chemically attached to carboxymethylcellulose.化学连接到羧甲基纤维素上的无花果蛋白酶的制备及其性质
Biochem J. 1966 Feb;98(2):420-5. doi: 10.1042/bj0980420.

由长链阴离子激活产生的磷脂酶D最佳pH值的变化。

A shift in the optimum pH of pospholipase D produced by activating long-chain anions.

作者信息

Quarles R H, Dawson R M

出版信息

Biochem J. 1969 May;112(5):795-9. doi: 10.1042/bj1120795.

DOI:10.1042/bj1120795
PMID:5821735
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1187786/
Abstract
  1. The activity of phospholipase D (phosphatidylcholine phosphatidohydrolase, EC 3.1.4.4) towards ultrasonically treated phosphatidylcholine or large phosphatidylcholine particles activated with ether was maximal near pH5, and there was little activity above pH6. 2. When the enzyme was activated by the addition of phosphatidic acid to large phosphatidylcholine particles the pH optimum was shifted to pH6.5 irrespective of the amount of activator added. 3. When the enzyme was activated with low concentrations of dodecyl sulphate the pH optimum was 5.5 with little activity above pH6. With higher concentrations of dodecyl sulphate the pH-activity profile was shifted upwards towards a pH optimum of 6.5-6.6, the magnitude of the shift depending on the extent of the hydrolysis. 4. The shifts in the pH-activity profiles cannot be correlated with changes in the ;surface pH' of the substrate particles calculated from the measurement of their zeta-potentials (electrophoretic mobilities).
摘要
  1. 磷脂酶D（磷脂酰胆碱磷脂水解酶，EC 3.1.4.4）对经超声处理的磷脂酰胆碱或用乙醚活化的大磷脂酰胆碱颗粒的活性在pH5附近最大，在pH6以上活性很低。2. 当通过向大磷脂酰胆碱颗粒中添加磷脂酸来激活该酶时，无论添加的激活剂数量如何，最适pH都移至pH6.5。3. 当用低浓度的十二烷基硫酸盐激活该酶时，最适pH为5.5，在pH6以上活性很低。使用较高浓度的十二烷基硫酸盐时，pH-活性曲线向上移动，最适pH为6.5 - 6.6，移动幅度取决于水解程度。4. pH-活性曲线的移动与根据底物颗粒的ζ电位（电泳迁移率）测量计算出的底物颗粒“表面pH”的变化无关。