化学连接到羧甲基纤维素上的无花果蛋白酶的制备及其性质
The preparation and properties of ficin chemically attached to carboxymethylcellulose.
作者信息
Hornby W E, Lilly M D, Crook E M
出版信息
Biochem J. 1966 Feb;98(2):420-5. doi: 10.1042/bj0980420.
Abstract
- Purified ficin has been coupled to four CM-celluloses by reaction with their acid azide derivatives. Insoluble products containing 1.8-4.7mg. of ficin/100mg. of product and retaining 8.0-12.0% of the free enzyme's esterase activity have been obtained. 2. The amount of bound ficin in these preparations is dependent on the degree of carboxymethyl substitution of the CM-cellulose to which the ficin is attached. 3. A shift of the alkaline limb of the pH-activity curve of ficin when chemically attached to CM-cellulose has been shown. 4. Only a small loss has been observed in the enzymic activity of these products when stored at 2 degrees for 4 months. They are more resistant than free enzyme to heat denaturation. 5. Columns of CM-cellulose-ficin have been packed. The degree of hydrolysis of perfused substrate has been measured for different flow rates through the column. 6. The properties of these derivatives have been discussed.
摘要
- 纯化的无花果蛋白酶通过与四种羧甲基纤维素的酸叠氮衍生物反应而与之偶联。已获得了不溶性产物，其每100毫克产物含1.8 - 4.7毫克无花果蛋白酶，并保留了游离酶酯酶活性的8.0 - 12.0%。2. 这些制剂中结合的无花果蛋白酶量取决于与无花果蛋白酶相连的羧甲基纤维素的羧甲基取代程度。3. 已表明，当无花果蛋白酶化学连接到羧甲基纤维素上时，其pH - 活性曲线的碱性部分会发生偏移。4. 这些产物在2℃下储存4个月后，酶活性仅有少量损失。它们比游离酶更耐热变性。5. 已装填了羧甲基纤维素 - 无花果蛋白酶柱。针对通过柱子的不同流速，测定了灌注底物的水解程度。6. 已对这些衍生物的性质进行了讨论。
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