Barr R D, Benton S G
Arch Pathol Lab Med. 1979 Jun;103(6):293-6.
The recovery of monocytes, following density sedimentation of human peripheral blood, was previously observed to be greater than 100%. An explanation was sought in the present study. No evidence for erroneous estimation of these cells was found. Rather, the data suggest that lymphocytes may take on the appearance of monocytes as a consequence of cytocentrifugation. This phenomenon does not appear to be influenced by the osmolality or viscosity of the suspending medium, nor by the process of sedimentation. Moreover, polymorphonuclear leukocytes do not seem to participate in the process of transformation, which may depend instead on the transfer of cytoplasm from monocytes to lymphocytes.
先前观察到，人类外周血经密度沉降后单核细胞的回收率大于100%。本研究旨在寻求一种解释。未发现这些细胞估计错误的证据。相反，数据表明，淋巴细胞可能由于细胞离心涂片而呈现出单核细胞的外观。这种现象似乎不受悬浮介质的渗透压或粘度的影响，也不受沉降过程的影响。此外，多形核白细胞似乎不参与转化过程，转化过程可能反而依赖于细胞质从单核细胞向淋巴细胞的转移。
