Gadeberg O V, Rhodes J M, Larsen S O
Acta Haematol. 1982;68(4):313-6. doi: 10.1159/000206999.
Differential counts of smears and cytospins of full blood, dextran fractionated blood leukocytes and metrizoate/Ficoll fractionated mononuclear blood cells were compared. Frequencies of lymphocytes decreased in cytospins as compared with smears. The monocyte/lymphocyte ratios were 0.24 in smears of full blood and 0.22, 0.31, 0.15 and 0.39 in smears and cytospins of dextran supernatant cells and metrizoate/Ficoll interface cells, respectively. Less than 5% of cells were broken in all preparations. The slide to slide variation was larger in preparations of fractionated cells than in smears of full blood. Cells were evenly distributed in mononuclear cytospins.
对全血涂片和细胞离心涂片、葡聚糖分级分离的血液白细胞以及泛影葡胺/聚蔗糖分级分离的单核血细胞进行了分类计数比较。与涂片相比，细胞离心涂片中淋巴细胞的频率降低。全血涂片的单核细胞/淋巴细胞比率为0.24，葡聚糖上清液细胞涂片和细胞离心涂片以及泛影葡胺/聚蔗糖界面细胞涂片和细胞离心涂片的该比率分别为0.22、0.31、0.15和0.39。所有制剂中细胞破碎率均低于5%。分级分离细胞制剂的玻片间差异比全血涂片的大。细胞在单核细胞离心涂片中分布均匀。
