Warocquier R, Samaille J, Green M
J Virol. 1969 Oct;4(4):423-8. doi: 10.1128/JVI.4.4.423-428.1969.
The synthesis of infectious virus is reduced by 99% in adenovirus type 2-infected KB cells maintained at 42 C in suspension. Studies to delineate the steps in virus biosynthesis blocked at 42 C revealed the following. (i) The inhibition of ribosomal ribonucleic acid (RNA) synthesis and the block in the conversion of ribosomal RNA precursors to ribosomal RNA which normally occur in infected cells at 37 C was accentuated at 42 C. (ii) The same species of early and late viral messenger RNA were synthesized at 37 and 42 C. (iii) The shift from host cell deoxyribonucleic acid (DNA) synthesis to viral DNA replication occurred earlier at 42 C than at 37 C. These findings indicate that the thermosensitive block(s) in virion formation does not occur at the level of viral DNA replication or transcription of viral mRNA but probably involves the synthesis of late viral proteins or the maturation of the virion.
在悬浮状态下于42℃培养的腺病毒2型感染的KB细胞中，传染性病毒的合成减少了99%。为确定在42℃时病毒生物合成中受阻步骤所开展的研究揭示了以下情况。(i) 在42℃时，核糖体核糖核酸（RNA）合成的抑制以及核糖体RNA前体向核糖体RNA的转化受阻加剧，而这些情况在37℃的受感染细胞中通常会出现。(ii) 在37℃和42℃时合成的早期和晚期病毒信使RNA种类相同。(iii) 从宿主细胞脱氧核糖核酸（DNA）合成向病毒DNA复制的转变在42℃时比在37℃时发生得更早。这些发现表明，病毒粒子形成过程中的热敏性阻断并非发生在病毒DNA复制或病毒mRNA转录水平，而可能涉及晚期病毒蛋白的合成或病毒粒子的成熟。
