Scheidtmann K H, Ortin J, Doerfler W
Eur J Biochem. 1975 Oct 15;58(2):283-90. doi: 10.1111/j.1432-1033.1975.tb02374.x.
In human KB cells productively infected with adenovirus type 12, viral DNA replication starts between 12 and 14h postinfection. Virus-specific, polysome-associated mRNA was investigated early (6-8h) and late (26-28h) after infection. Most of the viral mRNA was polyadenylated and accounted for 0.46% and 24.1% of the mRNA synthesized early and late postinfection, respectively. The viral-specific mRNA isolated both early and late after infection falls into several distinct size-classes, ranging in molecular weights between 0.3X10(6) and 1.5X10(6) for the early RNA and between 0.6X10(6) and 2.3X10(6) for the RNA synthesized late in the infection.
在被12型腺病毒有效感染的人KB细胞中，病毒DNA复制在感染后12至14小时开始。在感染后的早期（6 - 8小时）和晚期（26 - 28小时）对病毒特异性、多核糖体相关的mRNA进行了研究。大多数病毒mRNA是多聚腺苷酸化的，分别占感染后早期和晚期合成的mRNA的0.46%和24.1%。感染后早期和晚期分离出的病毒特异性mRNA分为几个不同的大小类别，早期RNA的分子量在0.3×10⁶至1.5×10⁶之间，感染后期合成的RNA的分子量在0.6×10⁶至2.3×10⁶之间。
