黄连素：与DNA结合物
Berberine: complex with DNA.
作者信息
Krey A K, Hahn F E
出版信息
Science. 1969 Nov 7;166(3906):755-7. doi: 10.1126/science.166.3906.755.
PMID:5823315
Abstract
A complex of calf-thymus DNA with berberine sediments in the analytical ultracentrifuge. The DNA produced systematic changes in the absorption spectrum of berberine which suggest that single alkaloid molecules bind to DNA. Flow dichroism of purines and pyrimidines and of berberine in the complex with DNA had the same signs and magnitudes. Berberine shifted the thermal strand separation profile of DNA to higher temperatures. Therefore, the alkaloid forms a complex with DNA, probably by intercalation.
摘要
小檗碱与小牛胸腺DNA的复合物在分析超速离心机中会沉淀。DNA使小檗碱的吸收光谱发生系统性变化，这表明单个生物碱分子与DNA结合。嘌呤、嘧啶以及与DNA形成复合物的小檗碱的流动二色性具有相同的符号和大小。小檗碱将DNA的热链分离曲线移向更高温度。因此，该生物碱可能通过嵌入作用与DNA形成复合物。
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