Sefidabi Reyhaneh, Mortazavi Pejman, Hosseini Saeed
Department of Pathology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran 1477893855, Iran.
Biomed Rep. 2017 Jan;6(1):95-98. doi: 10.3892/br.2016.809. Epub 2016 Nov 11.
Canine mammary gland tumors are the most frequent cause of cancer in female dogs. Numerous studies using cancer cell lines and clinical trials have indicated that various natural products and antioxidants reduce or possibly prevent the development of cancer. Berberine (BBR), the most important alkaloid in the Berberidaceae, which exerts a wide range of pharmacological and biochemical effects, has drawn much attention due to its particularly high antitumor activity and in animal studies. The aim of the present study was to investigate the antiproliferative effect of BBR against a canine mammary gland carcinoma cell line (CF41.Mg) . CF41.Mg cells were cultured in RPMI-1640 medium containing 10% heat inactived fetal bovine serum (FBS) and 100 mg/ml peniciline-streptomycin. Subsequently the cells were treated with different concentrations of BBR chloride (10, 25, 50, 100 and 200 µM) at a density of 12,000 cells/well in 96-well plates. Following treatment, the MTT assay was used to detect cell viability after 24-, 48- and 72-h incubations at 37°C with 5% CO. The results indicated that BBR inhibited proliferation of canine mammary gland carcinoma cells, as treatment with 100 µM BBR for 24 h resulted in a significant decrease in cell viability (P<0.005). As the present study demonstrated that BBR (10-200 µM) induced cancer cell death, it is proposed that BBR may serve as a candidate agent against canine mammary tumor cells via its antiproliferative activity.
犬乳腺肿瘤是雌性犬类最常见的癌症病因。众多使用癌细胞系的研究和临床试验表明，各种天然产物和抗氧化剂可减少或可能预防癌症的发生。小檗碱（BBR）是小檗科中最重要的生物碱，具有广泛的药理和生化作用，因其特别高的抗肿瘤活性在动物研究中备受关注。本研究的目的是探讨BBR对犬乳腺癌细胞系（CF41.Mg）的抗增殖作用。CF41.Mg细胞在含有10%热灭活胎牛血清（FBS）和100mg/ml青霉素-链霉素的RPMI-1640培养基中培养。随后，将细胞以12,000个细胞/孔的密度接种于96孔板中，并用不同浓度的氯化小檗碱（10、25、50、100和200μM）进行处理。处理后，采用MTT法检测在37℃、5%CO₂条件下孵育24、48和72小时后的细胞活力。结果表明，BBR抑制犬乳腺癌细胞的增殖，因为用100μM BBR处理24小时导致细胞活力显著下降（P<0.005）。由于本研究表明BBR（μM）可诱导癌细胞死亡，因此推测BBR可能通过其抗增殖活性成为对抗犬乳腺肿瘤细胞的候选药物。
