Guy D, Latner A L, Turner G A
Exp Cell Biol. 1979;47(4):312-9. doi: 10.1159/000162950.
A very simple, rapid and reproducible method has been developed for studying the interaction of lectins with the cell surface. This involves determining the number of adherent cells after shaking cell suspensions in Petri dishes which have had a lectin coupled to their surface using 1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide metho-p-toluene sulfonate. Using concanavalin A coupled to 60 mm diameter dishes and between 1.5 and 2 x 10(6) tumour cells, this adhesion reached a maximum after 10 min shaking. Maximum cell adhesion also varied according to the particular lectin used. Adhesion was absent or was very low if cells were shaken in untreated dishes, or in dishes coupled to bovine serum albumin, or in the presence of the lectin-specific sugar-competitor. Under conditions of maximum cell adhesion, the binding of two different lymphosarcoma lines to four different lectins was very similar, whereas the binding of a carcinoma line to these lectins was completely different from that observed for the lymphosarcomas.
已开发出一种非常简单、快速且可重复的方法来研究凝集素与细胞表面的相互作用。这涉及到在培养皿中摇晃细胞悬液后，测定贴壁细胞的数量，该培养皿的表面已使用对甲苯磺酸1-环己基-3-(2-吗啉代乙基)碳二亚胺偶联了凝集素。使用偶联在直径60毫米培养皿上的伴刀豆球蛋白A以及1.5至2×10⁶个肿瘤细胞，摇晃10分钟后这种黏附达到最大值。最大细胞黏附也因所使用的特定凝集素而异。如果在未处理的培养皿中、或在偶联了牛血清白蛋白的培养皿中、或在存在凝集素特异性糖竞争剂的情况下摇晃细胞，则不存在黏附或黏附非常低。在最大细胞黏附的条件下，两种不同的淋巴肉瘤细胞系与四种不同凝集素的结合非常相似，而一种癌细胞系与这些凝集素的结合与淋巴肉瘤所观察到的完全不同。
