从非转移性和转移性仓鼠淋巴肉瘤中分离出的细胞的表面特性。
Surface properties of cells isolated from non-metastasizing and metastasizing hamster lymphosarcomas.
作者信息
Guy D, Latner A L, Sherbet G V, Turner G A
出版信息
Br J Cancer. 1980 Dec;42(6):915-21. doi: 10.1038/bjc.1980.340.
Present evidence suggests that the cell surface has an important role in metastasis. To examine this idea further, the surface properties of single cells isolated from the primary growths of a liver-metastasizing (ML) and a non-metastasizing (NML) lymphosarcoma were compared for adhesion to cell monolayers, cytopherometry, isoelectric focusing, adhesion to immobilized lectins and surface labelling with lactoperoxidase-catalysed radioiodination. It was found that the ML cells had increased adhesion to 3 out of 4 of the monolayers studies; a lower overall surface charge but greater peripheral concentrations of charge; and increased surface expression of the fucose moiety. No consistent difference between the two cell types was detected in the electrophoretic pattern of the labelled surface proteins. These findings are discussed in the light of present knowledge of the cell surface, and it is concluded that the significance of any of the observed changes in relation to metastasis has yet to be established.
目前的证据表明，细胞表面在转移过程中起着重要作用。为了进一步研究这一观点，比较了从肝转移性（ML）和非转移性（NML）淋巴肉瘤的原发性生长物中分离出的单细胞的表面特性，包括对细胞单层的粘附、细胞光度测定、等电聚焦、对固定凝集素的粘附以及用乳过氧化物酶催化的放射性碘化进行表面标记。结果发现，ML细胞对所研究的4种单层细胞中的3种的粘附增加；总体表面电荷较低，但周边电荷浓度较高；岩藻糖部分的表面表达增加。在标记的表面蛋白的电泳图谱中，未检测到两种细胞类型之间的一致差异。根据目前对细胞表面的了解对这些发现进行了讨论，并得出结论，任何观察到的与转移相关的变化的意义尚未确定。
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