The identity and origin of oligosaccharides present in the faeces and urine of sick infants.

A systematic identification scheme, based on improved paper and thin-layer chromatography, acidic and enzymatic hydrolysis, and the reaction of carbohydrates with several location reagents, has been applied to the analysis of oligosaccharides present in the urine and faeces of sick children and the diets they are fed. The identity and origin of these oligosaccharides is described and their relevance to the diagnosis and treatment of children with suspected disorders of carbohydrate metabolism is discussed.