Rotella C M, Fani P, Tanini A L, Cicchi P, Toccafondi R S, Arcangeli P
J Endocrinol Invest. 1979 Jan-Mar;2(1):59-63. doi: 10.1007/BF03349276.
Basal adenylate cyclase activity of thyroid plasma membranes obtained from six patients with Graves' disease was slightly but not significantly lower than normal (83.3 +/- 13.9 pmol cAMP/10 min/mg of protein versus 120.9 +/- 19.5 pmol cAMP/10 min/mg of protein). In five of these patients the adenylate cyclase activity was stimulated by bovine TSH with an apparent Km value similar to that of normal thyroid (3.1 +/- 0.5 X 10-9 M versus 3.4 +/- 0.6 X 10-9 M). The response to prostaglandin E2 was also normal. In the sixth patient adenylate cyclase activity was stimulated by prostaglandin E2 but not by bovine TSH. The distribution of basal adenylate cyclase activity in various gradient layers was studied in two TSH-responsive patients. A relative increase of this activity was found in the denser layer when compared to normal thyroid tissue. This could be the expression of an altered ratio between the protein and lipid components of the plasma membranes in patients with Graves' disease.
从6例格雷夫斯病患者获取的甲状腺质膜的基础腺苷酸环化酶活性略低于正常水平，但差异无统计学意义（分别为83.3±13.9 pmol cAMP/10分钟/毫克蛋白和120.9±19.5 pmol cAMP/10分钟/毫克蛋白）。其中5例患者的腺苷酸环化酶活性受到牛促甲状腺激素（TSH）刺激，其表观米氏常数（Km）值与正常甲状腺相似（分别为3.1±0.5×10⁻⁹ M和3.4±0.6×10⁻⁹ M）。对前列腺素E2的反应也正常。在第6例患者中，腺苷酸环化酶活性受到前列腺素E2刺激，但不受牛TSH刺激。在2例对TSH有反应的患者中研究了基础腺苷酸环化酶活性在不同梯度层中的分布。与正常甲状腺组织相比，在较致密层中发现该活性相对增加。这可能是格雷夫斯病患者质膜蛋白和脂质成分比例改变的表现。
