Cohnen E, Flasch H, Heinz N, Hempelmann F W
Arzneimittelforschung. 1978;28(12):2179-82.
Partition coefficients (P) of 48 cardenolides were determined in octanol/water. Furthermore Rm-values of most of these substances were measured in different thin-layer chromatography systems (TLC) and related to P. Regression analysis revealed that the best linear fit of the data was obtained when Rm-values from reversed-phase TLC on octanol impregnated silica-gel layer as stationary phase and methanol/water as mobile phase were correlated with log P (r = 0.91). A better correlation between these two sets of data was obtained by restricting the calculations to groups of cardenolides with similar structural features (r = 0.96-0.98). The present results are showing that Rm-values from reversed-phase TLC--a rapid and reproducible method with many advantages over the tedious measurement of P--can be used to predict P.
在正辛醇/水体系中测定了48种强心苷的分配系数（P）。此外，还在不同的薄层色谱系统（TLC）中测量了这些物质中大多数的比移值（Rm），并将其与P相关联。回归分析表明，当以正辛醇浸渍硅胶层为固定相、甲醇/水为流动相的反相TLC的Rm值与log P相关时，数据的最佳线性拟合得以实现（r = 0.91）。通过将计算限制在具有相似结构特征的强心苷组中，这两组数据之间获得了更好的相关性（r = 0.96 - 0.98）。目前的结果表明，反相TLC的Rm值——一种快速且可重复的方法，相较于繁琐的P测量具有许多优势——可用于预测P。
