Desreumaux C, Dedonder E, Dewailly P, Sézille G, Fruchart J C
Arzneimittelforschung. 1979;29(10):1581-3.
The effects of different types of phospholipids on the lipoprotein lipase (LPL) and on the hepatic triglyceride lipase (TGLH) were studied by comparative in vitro incubation. Substrates were prepared by homogenization in glycerol of 3H-labelled triolein plus phosphatidylcholine (either dipalmitoyl PC, or ovolecithin or highly unsaturated PC, EPL Nattermann). Enzymes were obtained from rat adipose tissue, myocardium, lungs and liver and human adipose tissue and post-heparin plasma, respectively. The enzyme activities as determined in mmol/ml medium showed differences of highly statistical significance (p less than 0.001). In any tissue investigated the enzyme activities of LPL or TGLH followed the sequence: highly unsaturated PC (EPL) greater than ovo-lecithin greater than dipalmitoyl-lecithin. This was true for the enzymes of rat and human origin as well. It was concluded that the observed LPL and TGLH activation may account for the beneficial therapeutic effects of highly unsaturated phosphatidylcholine (EPL) in human hyperglyceridemias.
通过体外比较孵育研究了不同类型磷脂对脂蛋白脂肪酶（LPL）和肝甘油三酯脂肪酶（TGLH）的影响。底物是通过将3H标记的三油精与磷脂酰胆碱（二棕榈酰磷脂酰胆碱、卵黄卵磷脂或高度不饱和磷脂酰胆碱，纳特曼公司的EPL）在甘油中匀浆制备的。酶分别从大鼠脂肪组织、心肌、肺和肝脏以及人类脂肪组织和肝素后血浆中获得。以mmol/ml培养基测定的酶活性显示出具有高度统计学意义的差异（p小于0.001）。在所研究的任何组织中，LPL或TGLH的酶活性遵循以下顺序：高度不饱和磷脂酰胆碱（EPL）＞卵黄卵磷脂＞二棕榈酰卵磷脂。这对于大鼠和人类来源的酶也是如此。得出的结论是，观察到的LPL和TGLH激活可能解释了高度不饱和磷脂酰胆碱（EPL）对人类高甘油三酯血症的有益治疗作用。
