哺乳期大鼠饮食中反式脂肪酸变化对乳腺、肝脏和脂肪组织中脂蛋白脂肪酶活性的影响。

Effect of variation of trans-fatty acid in lactating rats' diet on lipoprotein lipase activity in mammary gland, liver, and adipose tissue.

作者信息

Assumpção Renata Pereira, dos Santos Flávia Duarte, de Mattos Machado Andrade Priscila, Barreto Giselle Freire, das Graças Tavares do Carmo Maria

机构信息

Instituto de Nutrição Josué de Castro, Universidade Federal do Rio de Janeiro, Brazil.

出版信息

Nutrition. 2004 Sep;20(9):806-11. doi: 10.1016/j.nut.2004.05.004.

DOI:10.1016/j.nut.2004.05.004

PMID:15325692

Abstract

OBJECTIVE

Lactation is associated with an increase in lipoprotein lipase (LPL) activity in the mammary gland (MG) and a decrease in adipose tissue because lactation redirects circulating substrates to the MG for milk synthesis. We investigated the effects of different dietary contents of trans-fatty acid (TFA) on LPL activity in maternal tissues and fatty acid composition in milk.

METHODS

Lactating rats were fed semisynthetic isocaloric diets containing 7% soy oil (control), 7% partially hydrogenated vegetable oil (7%-PHVO), 5% PHVO plus 2% soy oil (5%-PHVO), or 3.5% PHVO plus 3.5% soy oil (3.5%-PHVO). On lactation day 14, animals were decapitated and MG, liver, and parametrial adipose tissue were extracted to determine total lipid contents, percentages of TFA, and LPL activity. Milk lipid composition was examined by gas chromatographic analysis of the gastric content of 14-d-old suckling pups.

RESULTS

Maternal consumption of TFA increased dietary TFA incorporation in MG and liver and decreased it in parametrial adipose tissue. Diets with higher trans concentrations (7%-PHVO) significantly increased lipid content in the MG, and all groups fed trans-based diets showed significant increases in LPL activity in the MG. Although LPL increased in the MG, milk of rats fed TFA-based diets had significant decreases in the percentage of essential fatty acids.

CONCLUSIONS

TFA intake during lactation alters maternal lipid metabolism and the percentage of essential fatty acids in milk; therefore, it is important to alert the population to avoid excessive intake of TFAs during lactation.

摘要

目的

哺乳期与乳腺中脂蛋白脂肪酶（LPL）活性增加及脂肪组织减少有关，因为哺乳期会将循环底物重新导向乳腺用于乳汁合成。我们研究了不同反式脂肪酸（TFA）饮食含量对母体组织中LPL活性及乳汁脂肪酸组成的影响。

方法

给哺乳期大鼠喂食含7%大豆油（对照）、7%部分氢化植物油（7%-PHVO）、5% PHVO加2%大豆油（5%-PHVO）或3.5% PHVO加3.5%大豆油（3.5%-PHVO）的半合成等热量饮食。在哺乳期第14天，将动物断头，提取乳腺、肝脏和子宫旁脂肪组织以测定总脂质含量、TFA百分比和LPL活性。通过对14日龄哺乳幼崽胃内容物进行气相色谱分析来检测乳汁脂质组成。