Martin R B, Morlino V J
Science. 1965 Oct 22;150(3695):493. doi: 10.1126/science.150.3695.493.
The carbon-bound hydrogen atoms of tyrosine that exchange with solvent protons in strongly acid solutions at about 100 degrees C are not the methylene hydrogen atoms but a pair on the aromatic ring. Of the two pairs of protons on the aromatic ring, observed in the proton magnetic resonance spectra, the pair at higher field undergoes exchange in 2.4N DCI at 100 degrees C. Other hydrogen atoms, attached either to aliphatic or aromatic carbon atoms, exhibit no noticeable exchange under the same conditions. From a chemicalshift analysis the exchanging protons are assigned as those ortho to the hydroxyl group on the aromatic ring.
在约100℃的强酸溶液中与溶剂质子发生交换的酪氨酸的碳键合氢原子，不是亚甲基氢原子，而是芳香环上的一对氢原子。在质子磁共振谱中观察到的芳香环上的两对质子中，处于较高场的那一对质子在100℃的2.4N DCI中发生交换。在相同条件下，与脂肪族或芳香族碳原子相连的其他氢原子没有明显的交换现象。通过化学位移分析，将发生交换的质子确定为芳香环上羟基邻位的质子。
