Cameron D G, Casal H L, Mantsch H H
J Biochem Biophys Methods. 1979;1(1):21-36. doi: 10.1016/0165-022x(79)90043-5.
Fourier transform infrared transmission spectroscopy is presented as a technique with great potential for the study of aqueous membrane preparations. The methodology of sample preparation, spectra recording and data reduction is outlined. Spectral parameters are derived from FT-IR difference spectra which provide an extremely sensitive means to monitor the temperature-dependent behavior of individual vibrational modes in model and natural membranes.