通过子宫液或β-淀粉酶在体外使精子获能。
Sperm capacitation by uterine fluid or beta-amylase in vitro.
作者信息
Kirton K T, Hafs H D
出版信息
Science. 1965 Oct 29;150(3696):618-9. doi: 10.1126/science.150.3696.618.
PMID:5837101
Abstract
Rabbit sperm developed the capacity to fertilize ova when incubated in utero or in vitro in fluids from estrous uteri. Incubation of sperm with beta-amylase in phosphate-buffered Locke's solution also resulted in capacitation. The data suggest that sperm capacitation involves enzymatic alteration of carbo-hydrate-containing seminal macromolecules which coat sperm and inhibit fertilization.
摘要
当兔精子在发情期子宫内或体外的子宫液中孵育时，它们就会发育出使卵子受精的能力。在磷酸盐缓冲的洛克溶液中，精子与β-淀粉酶一起孵育也会导致获能。数据表明，精子获能涉及到覆盖精子并抑制受精的含碳水化合物的精液大分子的酶促改变。
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