Kanwar K C, Yanagimachi R, Lopata A
Fertil Steril. 1979 Mar;31(3):321-7.
Using zona-free hamster eggs and salt-stored human eggs for assessing the fertilizing capacity of human spermatozoa, the effects of human seminal plasma on fertilizing capacity of human spermatozoa were investigated. The persistent presence of seminal plasma prevented sperm attachment to and penetration into the zona pellucida and vitellus. A series of experiments with zona-free hamster eggs revealed that, once the spermatozoa were preincubated in a seminal plasma-free environment known to induce the acrosome reaction, the seminal plasma no longer interfered with sperm-egg fusion. The native seminal plasma appears to interfere with both the acrosome reaction and vigorous motility of the spermatozoa, and this could be the reason fertilization fails when the plasma is consistently present around the spermatozoa. The fertilization-disturbing factor or factors in the seminal plasma appear to be macromolecular substances or substances associated with macromolecules.
利用无透明带仓鼠卵和盐储存的人卵来评估人类精子的受精能力，研究了人类精浆对人类精子受精能力的影响。精浆的持续存在会阻止精子附着并穿透透明带和卵黄。一系列使用无透明带仓鼠卵的实验表明，一旦精子在已知能诱导顶体反应的无精浆环境中预孵育，精浆就不再干扰精卵融合。天然精浆似乎会干扰精子的顶体反应和活力，而这可能就是当精浆持续存在于精子周围时受精失败的原因。精浆中干扰受精的因素似乎是大分子物质或与大分子相关的物质。
