Nemer M, Infante A A
Science. 1965 Oct 8;150(3693):217-21. doi: 10.1126/science.150.3693.217.
Rapidly labeled RNA from four-cell embryos and blastulae of sea urchins was analyzed by sedimentation and for ability to form DNA-RNA hybrids. The RNA was derived from polyribosomes and from the "gel interphase," an extraction compartment resulting from treatment of whole embryos with phenol and known to be enriched with nuclei. The RNA from both sources displayed a high degree of structural complementarity to DNA. This DNA-like RNA of the polyribosomes sedimented in discrete classes, rather than in the sedimentation continuum demonstrable for the labeled RNA of the gel interphase. Thus messenger RNA appears to emerge in the cytoplasm in discrete size classes.
对海胆四细胞胚胎和囊胚中快速标记的RNA进行了沉降分析，并检测了其形成DNA-RNA杂交体的能力。这些RNA来源于多核糖体和“凝胶中间相”，“凝胶中间相”是用苯酚处理整个胚胎后产生的一个提取区室，已知富含细胞核。来自这两个来源的RNA都与DNA表现出高度的结构互补性。多核糖体的这种类DNA RNA以离散的类别沉降，而不是像凝胶中间相标记的RNA那样呈现连续的沉降分布。因此，信使RNA似乎以离散的大小类别出现在细胞质中。
