兔晶状体在有氧和无氧情况下的葡萄糖代谢
The metabolism of glucose by the rabbit lens in the presence and absence of oxygen.
作者信息
Van Heyningen R
出版信息
Biochem J. 1965 Aug;96(2):419-31. doi: 10.1042/bj0960419.
Abstract
- The effect of the absence of oxygen on the metabolism of [U-(14)C]glucose by the rabbit lens has been examined. 2. Protein-free extracts of incubated lens were subjected to electrophoresis followed by chromatography on paper; radioautographs showed 10 compounds substantially labelled. In the absence of oxygen, less radioactivity appeared in glucose, sorbitol, fructose, alanine, glutamic acid, glutathione and nucleotides, and more in lactic acid, alpha-glycerophosphate and glycerol. 3. The radioactivity of lens protein was about two to four times greater after aerobic than after anaerobic incubation. 4. The radioactivity of carbon dioxide was 2- to 4-fold greater after aerobic incubation than after anaerobic incubation. 5. After aerobic incubation of the lens, the radioactivity of all the labelled compounds was higher in the outer part (cortex) than in the inner part (nucleus). 6. Free glycerol has been found to be present in the lens. Its concentration, and that of alpha-glycerophosphate, has been measured in the lens of several species of animal.
摘要
- 研究了缺氧对兔晶状体[U-(14)C]葡萄糖代谢的影响。2. 将孵育后的晶状体无蛋白提取物进行电泳，然后在纸上进行色谱分析；放射自显影片显示有10种化合物有明显标记。在无氧条件下，葡萄糖、山梨醇、果糖、丙氨酸、谷氨酸、谷胱甘肽和核苷酸中的放射性较低，而乳酸、α-甘油磷酸和甘油中的放射性较高。3. 需氧孵育后晶状体蛋白的放射性比厌氧孵育后大约高两到四倍。4. 需氧孵育后二氧化碳的放射性比厌氧孵育后高2至4倍。5. 晶状体需氧孵育后，所有标记化合物的放射性在外层（皮质）比内层（核）更高。6. 已发现晶状体中存在游离甘油。已测定了几种动物晶状体中游离甘油及其α-甘油磷酸的浓度。
相似文献
1
The metabolism of glucose by the rabbit lens in the presence and absence of oxygen.兔晶状体在有氧和无氧情况下的葡萄糖代谢
Biochem J. 1965 Aug;96(2):419-31. doi: 10.1042/bj0960419.
2
The metabolism of amino acids in the bovine lens. Their oxidation as a source of energy.牛晶状体中氨基酸的代谢。它们作为能量来源的氧化过程。
Biochem J. 1973 Sep;136(1):67-75. doi: 10.1042/bj1360067.
3
Fructose, fructose 1-phosphate, and glyceraldehyde breakdown in carbohydrate metabolism.果糖、1-磷酸果糖和甘油醛在碳水化合物代谢中的分解。
Ophthalmic Res. 1983;15(1):19-23. doi: 10.1159/000265232.
4
The metabolism of D-glyceraldehyde by the lens.晶状体对D-甘油醛的代谢
Biochem J. 1969 Apr;112(2):211-20. doi: 10.1042/bj1120211.
5
Sorbitol production in the lens: a means of counteracting glucose-derived osmotic stress.晶状体中山梨醇的产生：一种对抗葡萄糖衍生的渗透压应激的方式。
Ophthalmic Res. 1986;18(5):313-20. doi: 10.1159/000265455.
6
Polyol pathway activity in streptozotocin-diabetic rat lens.链脲佐菌素诱导的糖尿病大鼠晶状体中的多元醇途径活性
Exp Eye Res. 1989 Jul;49(1):87-92. doi: 10.1016/0014-4835(89)90078-x.
7
Effect of glycolaldehyde, glyceraldehyde, and related compounds on anaerobic metabolism in the rabbit lens.乙醇醛、甘油醛及相关化合物对兔晶状体无氧代谢的影响。
Proc Soc Exp Biol Med. 1970 Jul;134(3):658-62. doi: 10.3181/00379727-134-34855.
8
Glucose metabolism in the calf lens.
Exp Eye Res. 1985 Apr;40(4):629-41. doi: 10.1016/0014-4835(85)90085-5.
9
The effect of prolonged elevated glucose levels on the phosphate metabolism of the rabbit lens in perfused organ culture.
Exp Eye Res. 1986 Sep;43(3):329-41. doi: 10.1016/s0014-4835(86)80070-7.
10
Depression of reducing-equivalent translocation from substrate to oxygen in hepatic metabolism of sorbitol or glycerol in hypothyroidism.
Eur J Biochem. 1974 Oct 1;48(1):45-52. doi: 10.1111/j.1432-1033.1974.tb03741.x.
引用本文的文献
1
[The dependence of acid-base balance in the aqueous humor on carbon dioxide and oxygen diffusion through the cornea (author's transl)].房水中酸碱平衡对二氧化碳和氧气通过角膜扩散的依赖性（作者译）
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1980;214(2):101-5. doi: 10.1007/BF00572788.
2
[Polyphosphate nucleosides of crystallin. Their development during differentiation, aging and galactose overload].[晶状体的多磷酸盐核苷。它们在分化、衰老和半乳糖超载过程中的发展]
Doc Ophthalmol. 1970 Dec 21;29(1):1-126. doi: 10.1007/BF02346234.
3
[Amount of CO 2 produced by surviving lens epithelium].
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1971;183(2):137-9. doi: 10.1007/BF00407178.
4
[Citric acid content of lenses and single lens parts of different old bovines].[不同老龄牛晶状体及单个晶状体部分的柠檬酸含量]
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1969;179(1):32-40. doi: 10.1007/BF00410485.
5
The metabolism of glutamate, aspartate and alanine in the bovine lens.牛晶状体中谷氨酸、天冬氨酸和丙氨酸的代谢
Biochem J. 1971 Oct;124(5):72P-73P. doi: 10.1042/bj1240072p.
6
The metabolism of amino acids in the bovine lens. Their oxidation as a source of energy.牛晶状体中氨基酸的代谢。它们作为能量来源的氧化过程。
Biochem J. 1973 Sep;136(1):67-75. doi: 10.1042/bj1360067.
7
The role of respiration in the energy metabolism of the bovine lens.呼吸作用在牛晶状体能量代谢中的作用。
Biochem J. 1972 Sep;129(2):507-9. doi: 10.1042/bj1290507.
8
[The aconitase activity in certain parts of calf and bovine lenses].[小牛和牛晶状体某些部位的乌头酸酶活性]
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1972;184(2):151-4. doi: 10.1007/BF02390261.
9
The metabolism of D-glyceraldehyde by the lens.晶状体对D-甘油醛的代谢
Biochem J. 1969 Apr;112(2):211-20. doi: 10.1042/bj1120211.
本文引用的文献
1
Biochemical and clinical changes in the rabbit lens during alloxan diabetes.四氧嘧啶糖尿病兔晶状体的生化及临床变化
Biochem J. 1950 May;46(5):575-8. doi: 10.1042/bj0460575.
2
Studies on the crystalline lens: technic for in vitro culture of crystalline lenses and observations.晶状体研究：晶状体体外培养技术及观察
Arch Ophthal. 1950 Jun;43(6):979-88.
3
Removal of acid by trioctylamine from samples for microbiological assay.用三辛胺从用于微生物测定的样品中去除酸。
Biochem J. 1951 Apr;48(4):487-90. doi: 10.1042/bj0480487.
4
Detection of sugars on paper chromatograms.纸色谱上糖的检测
Nature. 1950 Sep 9;166(4219):444-5. doi: 10.1038/166444b0.
5
Studies on the crystalline lens. III. Incorporation of glycine and serine in the proteins of lenses cultured in vitro.晶状体研究。III. 体外培养晶状体蛋白质中甘氨酸和丝氨酸的掺入。
AMA Arch Ophthalmol. 1950 Nov;44(5):651-8.
6
The utilization of pyruvate and its conversion to glutamate in calf lens.丙酮酸在小牛晶状体中的利用及其向谷氨酸的转化。
Exp Eye Res. 1961 Sep;1:53-9. doi: 10.1016/s0014-4835(61)80008-0.
7
Accumulation of amino acids by calf lens.小牛晶状体对氨基酸的积累。
Invest Ophthalmol. 1962 Jun;1:368-76.
8
THE INITIAL SUBCELLULAR SITE OF INCORPORATION OF HEXOSES INTO LIVER PROTEIN.己糖掺入肝蛋白的初始亚细胞位点。
J Biol Chem. 1964 Jun;239:1686-9.
9
ALPHA-GLYCEROPHOSPHATE METABOLISM IN MUSCLE UNDER AEROBIC AND HYPOXIC CONDITIONS.有氧和低氧条件下肌肉中的α-甘油磷酸代谢
Am J Physiol. 1964 Mar;206:599-602. doi: 10.1152/ajplegacy.1964.206.3.599.
10
FACTORS AFFECTING THE FORMATION OF SUGAR ALCOHOLS IN OCULAR LENS.影响眼晶状体中糖醇形成的因素。
Biochim Biophys Acta. 1963 Aug 13;74:340-50. doi: 10.1016/0006-3002(63)91377-5.
Zotero 插件