Ohrloff C, Zierz S, Hockwin O
Ophthalmic Res. 1983;15(1):19-23. doi: 10.1159/000265232.
Recent investigations showed that all enzymes that are involved in the catabolism of fructose via fructose 1-phosphate (F-1-p) are present in the bovine lens. The present studies were performed to provide information regarding the extent to which fructose, F-1-p and glyceraldehyde (GA) are actually catabolized via this pathway, and how they increase the lactate, alpha-glycerophosphate and glycerol concentrations. Incubation was performed with a homogenate of bovine lens equator. F-1-p, and particularly fructose + ATP, considerably increased lactate and alpha-glycerophosphate concentrations, while the glycerol concentration remained unchanged compared to controls. Lactate formation through GA was not observed; while small amounts of glycerol and alpha-glycerophosphate are formed, it seems that the major part is transformed to glycerate. The results show that the further breakdown of lenticular fructose occurs via F-1-p and that the phosphorylated C3 fragments (dihydroxyacetone phosphate) are transferred to the energy metabolism, which is not the case with GA.
最近的研究表明，所有通过1-磷酸果糖（F-1-p）参与果糖分解代谢的酶都存在于牛晶状体中。进行本研究是为了提供有关果糖、F-1-p和甘油醛（GA）通过该途径实际分解代谢的程度，以及它们如何增加乳酸、α-甘油磷酸和甘油浓度的信息。用牛晶状体赤道部的匀浆进行孵育。F-1-p，特别是果糖+ATP，显著增加了乳酸和α-甘油磷酸的浓度，而与对照组相比，甘油浓度保持不变。未观察到通过GA形成乳酸；虽然形成了少量甘油和α-甘油磷酸，但似乎大部分转化为甘油酸。结果表明，晶状体果糖的进一步分解通过F-1-p进行，磷酸化的C3片段（磷酸二羟丙酮）被转移到能量代谢中，而GA并非如此。
