Adams J F, Kennedy E H
J Clin Pathol. 1965 Nov;18(6):780-2. doi: 10.1136/jcp.18.6.780.
The radioactive material in urine after parenteral radioactive hydroxocobalamin is composed of radioactive cyanocobalamin, radioactive hydroxocobalamin, and a radioactive anionic complex. Studies in vitro suggest that these materials have a similar microbiological activity. Comparison of the amounts of radioactive and microbiologically active material in urine after parenteral radioactive hydroxocobalamin show a significant correlation both in the first and second 24 hours after injection showing that the amount of radioactivity is an acceptable measure of the loss of cobalamin in urine. The slope of the regression lines obtained from cobalamin-deficient and normal subjects implies that there is no equilibration of injected radioactive hydroxocobalamin and body stores: this may be due to the absence of hydroxocobalamin from tissues or to the fact that tissue hydroxocobalamin is bound and incapable of equilibration.
肠胃外注射放射性羟钴胺素后，尿液中的放射性物质由放射性氰钴胺素、放射性羟钴胺素和一种放射性阴离子复合物组成。体外研究表明，这些物质具有相似的微生物活性。肠胃外注射放射性羟钴胺素后，尿液中放射性物质与微生物活性物质含量的比较显示，在注射后的第一个24小时和第二个24小时均存在显著相关性，这表明放射性含量是衡量尿液中钴胺素流失的可接受指标。从钴胺素缺乏和正常受试者获得的回归线斜率表明，注射的放射性羟钴胺素与体内储存之间不存在平衡：这可能是由于组织中缺乏羟钴胺素，或者是由于组织中的羟钴胺素已结合且无法达到平衡。
