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Kinetics of the reaction of alpha-chymotrypsin with diisopropyl fluorophosphate, with measurements of hydrogen ion release, enzyme inhibition, and spectral changes of enzyme at 290 mu.

作者信息

Moon A Y, Sturtevant J M, Hess G P

出版信息

J Biol Chem. 1965 Nov;240(11):4204-12.

PMID:5845823
Abstract
摘要

相似文献

1
Kinetics of the reaction of alpha-chymotrypsin with diisopropyl fluorophosphate, with measurements of hydrogen ion release, enzyme inhibition, and spectral changes of enzyme at 290 mu.α-胰凝乳蛋白酶与二异丙基氟磷酸酯反应的动力学,包括氢离子释放量、酶抑制作用的测定以及在290μm处酶的光谱变化。
J Biol Chem. 1965 Nov;240(11):4204-12.
2
CHARACTERIZATION OF A REVERSIBLY FORMED ENZYME COMPLEX IN THE REACTION OF CHYMOTRYPSIN WITH DIISOPROPYL FLUOROPHOSPHATE.胰凝乳蛋白酶与二异丙基氟磷酸酯反应中可逆形成的酶复合物的表征
J Biol Chem. 1965 Feb;240:717-21.
3
Investigations of the chymotrypsin-catalyzed hydrolysis of specific substrates. II. Characterization of the spectral changes of the enzyme at 290 m-mu and determination of over-all enzyme-substrate dissociation constants.胰凝乳蛋白酶催化特定底物水解的研究。II. 酶在290毫微米处光谱变化的表征及酶-底物总解离常数的测定。
J Biol Chem. 1967 Sep 10;242(17):3963-72.
4
Activity and conformation of the alkaline form of delta-chymotrypsin studied by the specific acylation of isoleucine-16.通过异亮氨酸-16的特异性酰化作用研究δ-胰凝乳蛋白酶碱性形式的活性和构象。
Biochemistry. 1970 Sep 29;9(20):3902-13. doi: 10.1021/bi00822a008.
5
Chymotrypsin-substrate complexes. Hydrogen ion equilibria in chymotrypsin and diisopropylphosphoryl-chymotrypsin.
Biochem Biophys Res Commun. 1964;14:313-7. doi: 10.1016/s0006-291x(64)80002-4.
6
Prototropic equilibrium of the reaction of diisopropylphosphofluoridate with alpha-chymotrypsin.二异丙基氟磷酸酯与α-糜蛋白酶反应的质子转移平衡
Biochim Biophys Acta. 1968 Sep 10;168(1):73-8. doi: 10.1016/0005-2795(68)90235-3.
7
The combination of chymotrypsin and chymotrypsinogen with fluorescent substrates and inhibitors for chymotrypsin.
Biochemistry. 1966 Apr;5(4):1413-25. doi: 10.1021/bi00868a040.
8
The inhibition of porcine pancreas kallikrein by di-isopropyl-fluorophosphate. Kinetics, stoichiometry, and nature of the group phosphorylated.
Eur J Biochem. 1969 Oct;10(3):419-25. doi: 10.1111/j.1432-1033.1969.tb00706.x.
9
Mode of inhibition of chymotrypsin by diisopropyl fluorophosphate. II. Introduction of isopropyl and elimination of fluorine as hydrogen fluoride.二异丙基氟磷酸对胰凝乳蛋白酶的抑制方式。II. 异丙基的引入及氟以氟化氢形式的消除。
J Biol Chem. 1950 Jul;185(1):209-20.
10
Alpha-chymotrypsin-catalyzed hydrolysis of some carbonate esters.
J Pharm Sci. 1968 Feb;57(2):282-7. doi: 10.1002/jps.2600570208.

引用本文的文献

1
The interaction of alpha-chymotrypsin with isosteric substrates of different charge type.α-胰凝乳蛋白酶与不同电荷类型的等排底物的相互作用。
Biochem J. 1967 Sep;104(3):762-6. doi: 10.1042/bj1040762.