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Investigations of the chymotrypsin-catalyzed hydrolysis of specific substrates. II. Characterization of the spectral changes of the enzyme at 290 m-mu and determination of over-all enzyme-substrate dissociation constants.

作者信息

Himoe A, Brandt K G, Hess G P

出版信息

J Biol Chem. 1967 Sep 10;242(17):3963-72.

PMID:6037554
Abstract
摘要

相似文献

1
Investigations of the chymotrypsin-catalyzed hydrolysis of specific substrates. II. Characterization of the spectral changes of the enzyme at 290 m-mu and determination of over-all enzyme-substrate dissociation constants.胰凝乳蛋白酶催化特定底物水解的研究。II. 酶在290毫微米处光谱变化的表征及酶-底物总解离常数的测定。
J Biol Chem. 1967 Sep 10;242(17):3963-72.
2
Investigations of the chymotrypsin-catalyzed hydrolysis of specific substrates. 3. Determination of individual rate constants and enzyme-substrate binding constants for specific amide and ester substrates.胰凝乳蛋白酶催化特定底物水解的研究。3. 特定酰胺和酯底物的各个速率常数及酶-底物结合常数的测定。
J Biol Chem. 1967 Sep 10;242(17):3973-82.
3
Investigations of the chymotrypsin-catalyzed hydrolysis of specific substrates. V. Determination of pre-steady state kinetic parameters for specific substrate esters by stopped flow techniques.胰凝乳蛋白酶催化特定底物水解的研究。V. 用停流技术测定特定底物酯的稳态前动力学参数。
J Biol Chem. 1971 May 10;246(9):2918-25.
4
CHARACTERIZATION OF A REVERSIBLY FORMED ENZYME COMPLEX IN THE REACTION OF CHYMOTRYPSIN WITH DIISOPROPYL FLUOROPHOSPHATE.胰凝乳蛋白酶与二异丙基氟磷酸酯反应中可逆形成的酶复合物的表征
J Biol Chem. 1965 Feb;240:717-21.
5
Investigations of the chymotrypsin-catalyzed hydrolysis of specific substrates. I. The pH dependence of the catalytic hydrolysis of N-acetyl-L-tryptophanamide by three forms of the enzyme at alkaline pH.胰凝乳蛋白酶催化特定底物水解的研究。I. 三种形式的酶在碱性pH条件下对N-乙酰-L-色氨酸酰胺催化水解的pH依赖性。
J Biol Chem. 1967 Mar 10;242(5):919-29.
6
Kinetics of the reaction of alpha-chymotrypsin with diisopropyl fluorophosphate, with measurements of hydrogen ion release, enzyme inhibition, and spectral changes of enzyme at 290 mu.α-胰凝乳蛋白酶与二异丙基氟磷酸酯反应的动力学,包括氢离子释放量、酶抑制作用的测定以及在290μm处酶的光谱变化。
J Biol Chem. 1965 Nov;240(11):4204-12.
7
Mechanism of the -chymotrypsin-catalyzed hydrolysis of amides. pH dependence of k c and K m . Kinetic detection of an intermediate.α-胰凝乳蛋白酶催化酰胺水解的机制。kcat和Km的pH依赖性。中间体的动力学检测。
J Am Chem Soc. 1971 Dec 15;93(25):7079-87. doi: 10.1021/ja00754a066.
8
[Kinetics of alpha-chymotrypsin catalyzed hydrolysis in equilibrium. II. Comparison of ester and amide substrates].[α-胰凝乳蛋白酶催化水解反应的动力学平衡。II. 酯和酰胺底物的比较]
Mol Biol (Mosk). 1977 Sep-Oct;11(5):1155-9.
9
On the spontaneous and alpha-chymotrypsin-catalyzed hydrolysis of 4-cis-benzylidene-2-phenyloxazolin-5-one. Catalysis by tris (hydroxymethyl) aminomethane.关于4-顺式亚苄基-2-苯基恶唑啉-5-酮的自发水解及α-胰凝乳蛋白酶催化水解。三(羟甲基)氨基甲烷的催化作用。
Biochemistry. 1969 May;8(5):1975-83. doi: 10.1021/bi00833a030.
10
Chymotrypsin-catalyzed phenyl ester hydrolysis. Evidence for electrophilic assistance on carbonyl oxygen.胰凝乳蛋白酶催化的苯基酯水解。羰基氧上亲电辅助作用的证据。
Biochemistry. 1970 Aug 18;9(17):3383-90. doi: 10.1021/bi00819a014.

引用本文的文献

1
Benzamidine as a spectroscopic probe for the primary specificity subsite of trypsin-like serine proteinases. A case for BPTI binding to bovine beta-trypsin.苯甲脒作为类胰蛋白酶丝氨酸蛋白酶一级特异性亚位点的光谱探针。关于抑肽酶与牛β-胰蛋白酶结合的一个实例。
Mol Cell Biochem. 1984 Sep;64(2):139-44. doi: 10.1007/BF00224770.