Mitani M, Iino T
J Bacteriol. 1965 Oct;90(4):1096-101. doi: 10.1128/jb.90.4.1096-1101.1965.
Mitani, Michiko (National Institute of Genetics, Mishima, Japan), and Tetsuo Iino. Electron microscopy of bundled flagella of the curly mutant of Salmonella abortivoequina. J. Bacteriol. 90:1096-1101. 1965.-The arrangement of flagella was observed by dark-field and electron microscopy in three strains of Salmonella abortivoequina, namely, normal flagellar, curly flagellar, and paralyzed curly flagellar strains. With dark-field microscopy, bundled flagella could be seen in 5 to 10% of actively moving normal or curly mutant cells. Under the electron microscope, a great many bundled flagella were observed in the curly mutant strain, but in the normal strain most of the flagella were dissociated or the bundles were rather loose and irregular. Normal flagella seem to separate easily during the process of preparation, but not the curly ones. Single flagella were found to run parallel with each other and to form a bundle consisting of five or more flagella; the bundle was spirally gyrating, with the characteristic flagellar wave. It is thought that the bundle observed with the electron microscope corresponds to that observed under the dark-field microscope. Further, the marked decrease of bundle formation in the paralyzed curly mutant cells suggests that bundle formation is not caused by curly flagellar structure per se, but corresponds to the mode of locomotion of peritrichously flagellated bacteria.
三宅美智子（日本三岛国立遗传学研究所）和饭野哲夫。马流产沙门氏菌卷曲突变体束状鞭毛的电子显微镜观察。《细菌学杂志》90:1096 - 1101。1965年。——通过暗视野和电子显微镜观察了马流产沙门氏菌的三个菌株的鞭毛排列情况，即正常鞭毛菌株、卷曲鞭毛菌株和麻痹卷曲鞭毛菌株。在暗视野显微镜下，在5%至10%活跃运动的正常或卷曲突变体细胞中可以看到束状鞭毛。在电子显微镜下，在卷曲突变菌株中观察到大量束状鞭毛，但在正常菌株中，大多数鞭毛是分离的，或者束相当松散且不规则。正常鞭毛在制备过程中似乎很容易分离，但卷曲鞭毛则不然。发现单根鞭毛相互平行排列，形成由五根或更多鞭毛组成的束；该束呈螺旋状旋转，带有特征性的鞭毛波。据认为，电子显微镜下观察到的束与暗视野显微镜下观察到的束相对应。此外，麻痹卷曲突变体细胞中束形成的显著减少表明，束形成不是由卷曲鞭毛结构本身引起的，而是与周生鞭毛细菌的运动方式相对应。
