一种在沙门氏菌中分离鞭毛形态突变体的简单方法。

A simple method for the isolation of flagellar shape mutants in Salmonella.

作者信息

Fujita H, Yamaguchi S, Taira T, Iino T

出版信息

J Gen Microbiol. 1981 Jul;125(1):213-6. doi: 10.1099/00221287-125-1-213.

Abstract

During prolonged static cultivation of liquid cultures of Salmonella strains with normal flagella, flagellar shape mutants occurred spontaneously and their proportion in the cultures increased so that they were easily isolated by spreading dilutions of the cultures on semisolid medium. By this method various types of flagellar shape mutants, including curly, straight and polymorphous mutants, were obtained.

摘要

在用正常鞭毛的沙门氏菌菌株进行液体培养的长时间静置培养过程中，鞭毛形状突变体自发出现，且它们在培养物中的比例增加，从而通过将培养物稀释液涂布在半固体培养基上可轻松分离出这些突变体。通过这种方法获得了各种类型的鞭毛形状突变体，包括卷曲、笔直和多形突变体。