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[采用染色体差异染色法对人类细胞系(CaVe和HeLa)进行核型分析]

[Karyotypic analysis of human cell lines (CaVe and HeLa) by the method of differential staining of chromosomes].

作者信息

Kulagina O E, Muzurenko N P, Pogosiants E E

出版信息

Vopr Virusol. 1975 Nov-Dec(6):677-81.

PMID:58478
Abstract

Karyological analysis of CaVe (human stomach cancer) and HeLa cell lines was carried out by means of differential Giemsa staining. In the CaVe cells 12 marker chromosomes were revealed, seven of them similar to those found in our HeLa cell strain. Four markers in our HeLa subline and seven in CaVe cells were similar to those of HeLa sublines described in literature. The authors have arrived at the conclusion that the CaVe line isolated in 1961 was contaminated and now represents a variant of HeLa cell line.

摘要

采用吉姆萨差异染色法对CaVe(人胃癌)细胞系和HeLa细胞系进行了核型分析。在CaVe细胞中发现了12条标记染色体,其中7条与我们的HeLa细胞株中发现的染色体相似。我们的HeLa亚系中的4条标记染色体和CaVe细胞中的7条标记染色体与文献中描述的HeLa亚系的标记染色体相似。作者得出结论,1961年分离出的CaVe细胞系已被污染,现在代表HeLa细胞系的一个变体。

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