Elliott A Y, Bronson D L, Cervenka J, Stein N, Fraley E E
Cancer Res. 1977 May;37(5):1279-89.
Characterization studies have been carried out on eight cell lines (253J, 192B, 639V, 647V, 486P, 575A,743E, and 751G) established from transitional cell cancers of the human urinary tract. Although subtle morphological differences exist among individual lines, each has an epithelial morphology and exhibits multilayering. The doubling times for the cells range from 20 to 56 hr, and at least a 1-to-3 split can be achieved when they are subcultured every 4th day. Karyotypic analysis revealed a hyperdiploid stemline for each cell line, and presence of a Y chromosome was confirmed by Q banding in five of the lines. The tumorigenic nature of the cell lines was demonstrated by their production of tumors in hamsters and confirmed by colony formation in agar. The transitional cell cancer lines were shown to be free of Mycoplasma, and their glucose-6-phosphate dehydrogenase mobility patterns and their Karyotypes prove that they are not HeLa cells.
对从人类泌尿道移行细胞癌建立的8个细胞系（253J、192B、639V、647V、486P、575A、743E和751G）进行了特性研究。尽管各个细胞系之间存在细微的形态差异，但每个细胞系都具有上皮形态并表现出多层结构。细胞的倍增时间为20至56小时，并且每4天传代培养时至少可实现1比3的分裂。核型分析显示每个细胞系都有一个超二倍体主干系，并且通过Q带分析在其中5个细胞系中证实了Y染色体的存在。细胞系的致瘤性通过它们在仓鼠体内产生肿瘤得到证明，并通过琼脂中的集落形成得到证实。这些移行细胞癌系被证明无支原体，并且它们的葡萄糖-6-磷酸脱氢酶迁移模式和核型证明它们不是HeLa细胞。
