Jentzsch K
Wien Klin Wochenschr. 1975 Dec 26;87(24):829-33.
One to eight week-old primary tissue cultures of human tumours (1 rhabomysarcoma, 1 renal adenocarcinoma, 1 hymphogranuloma, 2 melanomas) and cultures of chicken embryo cells and HeLa-cells were tested for their sensitivity to chemotherapeutic agents. The drugs tested were: vincristine, actinomycin D, adriblastine, bleomycin. The drug effect was recorded by registration of morphological changes and, in some cultures by a change in the 3-H-thymidine incorporation. The cultures showed a variable reaction to vincristine, a good response to actinomycin D and adriblastine and were uniformly resistant to bleomycin. Two cultures were pulse labelled with 3-H-thymidine to obtain some data in regard to their growth characteristics.
对人肿瘤的1至8周龄原代组织培养物（1例横纹肌肉瘤、1例肾腺癌、1例淋巴肉芽肿、2例黑色素瘤）以及鸡胚细胞和海拉细胞培养物进行了化疗药物敏感性测试。所测试的药物有：长春新碱、放线菌素D、阿霉素、博来霉素。通过记录形态学变化以及在某些培养物中通过3 - H - 胸苷掺入量的变化来记录药物效果。培养物对长春新碱表现出不同反应，对放线菌素D和阿霉素反应良好，对博来霉素均有抗性。对两种培养物用3 - H - 胸苷进行脉冲标记，以获取有关其生长特性的一些数据。
