Fischer E, Lovas M, Németh P
Acta Histochem. 1976;55(1):32-41.
From isolated botrydiosomes of Haemopis sanguisuga L. a pigment was extracted, separated by preparative paper chromatography and analysed chemically. Chromatographically the pigment was found to be composed of two fractions, which has been identified as zincporphyrin compounds, which are able to oxidize of diaminobenzidine in the presence of H2O2. As proved by ultrahistochemical method, the product of hte diaminobenzidine-H2O2 reaction is associated in the botryoid cells with the tubules foth of the endoplasmic reticulum and of botrydiosomes. The proboble functions of the zincporphyrins in the oxidizing processes are discussed.
从红蛭的分离出的葡萄状颗粒中提取出一种色素,通过制备性纸色谱法进行分离并进行化学分析。通过色谱分析发现该色素由两个组分组成,已鉴定为锌卟啉化合物,它们在过氧化氢存在下能够氧化二氨基联苯胺。超组织化学方法证明,二氨基联苯胺 - 过氧化氢反应的产物在葡萄状细胞中与内质网和葡萄状颗粒的小管相关联。讨论了锌卟啉在氧化过程中的可能功能。