Van't Hof J
J Cell Biol. 1965 Oct;27(1):179-89. doi: 10.1083/jcb.27.1.179.
The cell population kinetics of excised, cultured pea roots was studied with the use of tritiated thymidine and colchicine to determine (1) the influence of excision, (2) the influence of sucrose concentration, (3) the average mitotic cycle duration, and (4) the duration of mitosis and the G(1), S, and G(2) periods of interphase.(1) The results indicate that the process of excision causes a drop in the frequency of mitotic figures when performed either at the beginning of the culture period or after 100 hours in culture. This initial decrease in frequency of cell division is independent of sucrose concentration, but the subsequent rise in frequency of division, after 12 hours in culture, is dependent upon sucrose concentration. Two per cent sucrose maintains the shortest mitotic cycle duration. The use of colchicine indicated an average cycle duration of 20 hours, whereas the use of tritiated thymidine produced an average cycle duration of 17 hours.
利用氚标记的胸腺嘧啶核苷和秋水仙碱研究了切除并培养的豌豆根的细胞群体动力学，以确定：（1）切除的影响；（2）蔗糖浓度的影响；（3）平均有丝分裂周期持续时间；（4）有丝分裂持续时间以及间期的G1、S和G2期持续时间。（1）结果表明，无论是在培养期开始时还是在培养100小时后进行切除，切除过程都会导致有丝分裂细胞图像的频率下降。细胞分裂频率的这种初始下降与蔗糖浓度无关，但在培养12小时后，随后的分裂频率上升取决于蔗糖浓度。2%的蔗糖维持最短的有丝分裂周期持续时间。使用秋水仙碱表明平均周期持续时间为20小时，而使用氚标记的胸腺嘧啶核苷产生的平均周期持续时间为17小时。
