溴化氰对马心脏细胞色素c和马心脏肌红蛋白的作用。
The action of cyanogen bromide on horse-heart cytochrome c and horse-heart myoglobin.
作者信息
Black J A, Leaf G
出版信息
Biochem J. 1965 Sep;96(3):693-9. doi: 10.1042/bj0960693.
Abstract
- The effects of cyanogen bromide on horse-heart cytochrome c and horse-heart myoglobin have been investigated. Cytochrome c yielded four fragments, of which two were haemopeptides. The two colourless peptides had amino acid compositions corresponding to those that are expected, on the basis of the sequence proposed for horse-heart cytochrome c by Margoliash, Smith, Kreil & Tuppy (1961), from cleavage at both methionine residues. Of the two haemopeptides, one was isolated and shown to be that derived from cleavage at only one methionine residue, that nearer to the C-terminus of the peptide chain. 2. Myoglobin also gave four peptides, three of which accounted for the total amino acid content of the intact protein. The fourth fragment arose by cleavage at a single methionine residue, that nearer the C-terminus. Characterization of this fourth fragment made it possible to deduce the order of arrangement of the fragments in the intact molecule.
摘要
- 已经研究了溴化氰对马心脏细胞色素c和马心脏肌红蛋白的作用。细胞色素c产生了四个片段，其中两个是血红素肽。根据马戈利亚什、史密斯、克雷伊尔和图皮（1961年）提出的马心脏细胞色素c序列，这两个无色肽的氨基酸组成与在两个甲硫氨酸残基处裂解所预期的相符。在这两个血红素肽中，分离出了一个，结果表明它是仅在一个甲硫氨酸残基（更靠近肽链C端的那个）处裂解产生的。2. 肌红蛋白也产生了四个肽段，其中三个肽段占完整蛋白质的全部氨基酸含量。第四个片段是在一个甲硫氨酸残基（更靠近C端的那个）处裂解产生的。对这个第四个片段的表征使得推断完整分子中各片段的排列顺序成为可能。
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The action of cyanogen bromide on horse-heart cytochrome c and horse-heart myoglobin.溴化氰对马心脏细胞色素c和马心脏肌红蛋白的作用。
Biochem J. 1965 Sep;96(3):693-9. doi: 10.1042/bj0960693.
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引用本文的文献
1
The semisynthesis of fragments corresponding to residues 66-104 of horse heart cytochrome c.马心脏细胞色素c中对应于66 - 104位残基片段的半合成。
Biochem J. 1979 Apr 1;179(1):169-82. doi: 10.1042/bj1790169.
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Biochem J. 1952 Mar;50(5):707-12. doi: 10.1042/bj0500707.
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