Polysaccharide produced by the genus Pullularia. II. Trans-alpha-glucosidation by acetone cells of Pullularia.

Acetone cells of Pullularia sp. were incubated with maltose, and the saccharides produced were fractionated on a charcoal column. The fractions were subjected to paper-chromatographic analysis and enzymatic assay. By these methods, the saccharides have been shown to include glucose, maltose, maltotriose, panose, and the lower members of glucose polymers containing the 1,4- and 1,6-alpha-glucosidic linkages, but neither isomaltose nor dextrantriose. The use of glucose oxidase results in the formation of the higher members of glucose polymer.