Shinkarenko V S, Aleksandrov P N, Chernukh A M
Biull Eksp Biol Med. 1975 Jul;80(7):79-82.
A study was made of the efficacy of trypan blue, acridine orange, tetracycline and oxytetracycline for detection of tumour cells injected into the blood stream of rats. The cells were identified in the mesenteric microvessels by intravital microscopy. Fluorescence of fluorochromized cells was observed in the blue-violet (lambda max = 400 nm) and ultra-violet (lambda max = 365 nm) irradiation of the fluorescent lamp and in the laser irradiation (lambda = 337 nm). The cells stained with acridine orange had a higher fluorescence intensity and a more distinct structure than those labelled with tetracyclines. Identification of cells with trypan blue was more difficult. The fluorescent method of determination is rather simple and permits to indentify tumour cells directly in the blood stream.
对锥虫蓝、吖啶橙、四环素和土霉素检测注入大鼠血流中的肿瘤细胞的功效进行了一项研究。通过活体显微镜在肠系膜微血管中识别细胞。在用荧光灯进行蓝紫色（最大波长 = 400 纳米）和紫外线（最大波长 = 365 纳米）照射以及激光照射（波长 = 337 纳米）时，观察到了荧光染色细胞的荧光。与用四环素标记的细胞相比，用吖啶橙染色的细胞具有更高的荧光强度和更清晰的结构。用锥虫蓝鉴定细胞更困难。荧光测定法相当简单，能够直接在血流中识别肿瘤细胞。
