Edström A, Mattsson H
Brain Res. 1976 May 28;108(2):381-95. doi: 10.1016/0006-8993(76)90194-3.
The effects of sulfhydryl blocking agents have been studied on the rapid axonal transport in vitro of [3H]leucine-labelled proteins in the frog sciatic nerve. The transport was inhibited in the presence of low concentrations of N-ethylmaleimide (NEM) (greater than or equal to 10(-5) M), p-chloromercuribenzene sulfonic acid (PCMBS) (greater than 10(-5) M) or ions of heavy metals, Cd2+ (greater than or equal to 5 X 10(-5) M), Hg2+ (greater than or equal to 5 X 10(-6) M) and Cu2+(greater than or equal to 10(-4) M). Both the amount and the rate of transported radioactivity were reduced. Transport inhibiting concentrations of these agents also inhibited the binding of colchicine in rat brain or frog nerve supernatants. The amount of transported proteins was increased at an unchanged transport rate by a very low concentration of NEM (10(-6) M), PCMBS (10(-6) M) and Cd2+ (10(-6)M), which did not affect the binding of colchicine. The present results suggest that stimulation of axonal transport can be achieved through an interaction with sulfhydryl groups.
已研究了巯基阻断剂对青蛙坐骨神经中[3H]亮氨酸标记蛋白体外快速轴突运输的影响。在低浓度的N-乙基马来酰亚胺（NEM）（大于或等于10^(-5) M）、对氯汞苯磺酸（PCMBS）（大于10^(-5) M）或重金属离子Cd2+（大于或等于5×10^(-5) M）、Hg2+（大于或等于5×10^(-6) M）和Cu2+（大于或等于10^(-4) M）存在的情况下，运输受到抑制。运输的放射性数量和速率均降低。这些试剂的运输抑制浓度也抑制了秋水仙碱在大鼠脑或青蛙神经上清液中的结合。极低浓度的NEM（10^(-6) M）、PCMBS（10^(-6) M）和Cd2+（10^(-6)M）在运输速率不变的情况下增加了运输蛋白的数量，且这些浓度不影响秋水仙碱的结合。目前的结果表明，通过与巯基相互作用可以实现对轴突运输的刺激。
