委内瑞拉链霉菌噬菌体的纯化

Purification of Streptomyces venezuelae phage.

作者信息

Kolstad R A, Bradley S G

出版信息

J Bacteriol. 1964 May;87(5):1157-61. doi: 10.1128/jb.87.5.1157-1161.1964.

DOI:10.1128/jb.87.5.1157-1161.1964

PMID:5874537

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC277161/

Abstract

Kolstad, R. A. (University of Minnesota, Minneapolis), and S. G. Bradley. Purification of Streptomyces venezuelae phage. J. Bacteriol. 87:1157-1161. 1964.-Streptomyces venezuelae phage MSP8 was concentrated and purified by a combination of methods including dialysis against polyethylene glycol, partitioning between the two phases of aqueous polymer systems, gel filtration, chromatography on ECTEOLA-cellulose, and differential centrifugation. S. venezuelae phage MSP8 is 57% deoxyribonucleic acid and 43% protein. Its head is 55 by 70 mmu, and its tail is 10 by 150 mmu. Its dry weight is 250 mg per 10(15) plaqueforming units, and its density is 1.4. Phage MSP8 contains 15 mug of phosphorus and 40 mug of nitrogen per 10(12) particles. The ratio of light absorbancy at 260 to 280 mmu is 1.5. A mixture of two actinophages, MNP3 and MVP7, was separated by use of ECTEOLA-cellulose. In one fraction, 99% of the phage was MNP3; in another fraction, 99% of the phage was MVP7.

摘要

科尔斯塔德，R.A.（明尼苏达大学，明尼阿波利斯），以及S.G.布拉德利。委内瑞拉链霉菌噬菌体的纯化。《细菌学杂志》87:1157 - 1161。1964年。——委内瑞拉链霉菌噬菌体MSP8通过多种方法组合进行浓缩和纯化，这些方法包括用聚乙二醇透析、在水性聚合物系统的两相之间分配、凝胶过滤、ECTEOLA - 纤维素柱色谱以及差速离心。委内瑞拉链霉菌噬菌体MSP8含57%的脱氧核糖核酸和43%的蛋白质。其头部大小为55×70纳米，尾部大小为10×150纳米。其干重为每10¹⁵个噬菌斑形成单位250毫克，密度为1.4。噬菌体MSP8每10¹²个颗粒含15微克磷和40微克氮。在260至280纳米处的吸光度比值为1.5。利用ECTEOLA - 纤维素分离了两种放线菌噬菌体MNP3和MVP7的混合物。在一个组分中，99%的噬菌体是MNP3；在另一个组分中，99%的噬菌体是MVP7。