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天蓝链霉菌的一种温和噬菌体。

A temperate phage of Streptomyces azureus.

作者信息

Ogata S, Suenaga H, Hayashida S

出版信息

Appl Environ Microbiol. 1985 Jan;49(1):201-4. doi: 10.1128/aem.49.1.201-204.1985.

DOI:10.1128/aem.49.1.201-204.1985
PMID:2983606
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC238370/
Abstract

A new phage, SAt1, was isolated from soil on Streptomyces azureus ATCC 14921. This phage was able to lysogenize S. azureus. The percentage of lysogenic responses was ca. 10%. Electron microscopic observation showed that this phage belonged to group B of Bradley's morphological classification. The molecular mass of SAt1 DNA was ca. 24 megadaltons. The guanine-plus-cytosine content and the density of SAt1 DNA were ca. 71% and 1.724 g/cm3, respectively. A cleavage map of SAt1 DNA was constructed with restriction endonucleases BanIII, BglII, HindIII, and XbaI. Furthermore, some other characteristics of this phage were investigated.

摘要

从土壤中分离出一种新的噬菌体SAt1，其宿主为天蓝链霉菌ATCC 14921。该噬菌体能够使天蓝链霉菌溶源化。溶源化反应的百分比约为10%。电子显微镜观察表明，该噬菌体属于布拉德利形态分类中的B组。SAt1 DNA的分子量约为24兆道尔顿。SAt1 DNA的鸟嘌呤加胞嘧啶含量和密度分别约为71%和1.724 g/cm³。用限制性内切酶BanIII、BglII、HindIII和XbaI构建了SAt1 DNA的切割图谱。此外，还研究了该噬菌体的其他一些特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f4/238370/d02e33d1d688/aem00147-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f4/238370/d83c12bd1213/aem00147-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f4/238370/bb8f3c848d22/aem00147-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f4/238370/36fbc7fc31c3/aem00147-0219-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f4/238370/d02e33d1d688/aem00147-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f4/238370/d83c12bd1213/aem00147-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f4/238370/bb8f3c848d22/aem00147-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f4/238370/36fbc7fc31c3/aem00147-0219-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f4/238370/d02e33d1d688/aem00147-0220-a.jpg

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本文引用的文献

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Genetics and molecular biology of Streptomyces bacteriophages.链霉菌噬菌体的遗传学与分子生物学
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Appl Environ Microbiol. 1987 Dec;53(12):2708-13. doi: 10.1128/aem.53.12.2708-2713.1987.
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