Merkel J R, Traganza E D, Mukherjee B B, Griffin T B, Prescott J M
J Bacteriol. 1964 May;87(5):1227-33. doi: 10.1128/jb.87.5.1227-1233.1964.
Merkel, Joseph R. (Fort Johnson Marine Biological Laboratory, College of Charleston, Charleston, S.C.), Eugene D. Traganza, Barid B. Mukherjee, Travis B. Griffin, and J. M. Prescott. Proteolytic activity and general characteristics of a marine bacterium, Aeromonas proteolytica sp. n. J. Bacteriol. 87:1227-1233. 1964.-A highly proteolytic bacterial species was isolated from the alimentary canal of the marine borer, Limnoria. The morphological and biochemical characteristics of the organism indicated that it was a new Aeromonas species, for which the name A. proteolytica is proposed. When freshly isolated, the organism required seawater for growth; but, upon prolonged culture in the laboratory, it was able to grow in media of greatly reduced salt concentration, provided that relatively large amounts of peptone were supplied. Peptone or hydrolysates of casein were capable of supplying all organic nutrients required for growth and proteinase production. Certain individual amino acids were also able to furnish all energy, carbon, and nitrogen requirements. Inorganic nitrogen was utilized in the presence of citrate, but could not serve as the only source of nitrogen in the presence of glucose. The organism was facultatively anaerobic, but best growth and proteinase production occurred only with vigorous aeration. The amount of growth obtained in 24 hr increased rapidly as the incubation temperature was increased up to a maximum of 40 C, but no growth occurred at 42 C.
默克尔，约瑟夫·R.（查尔斯顿学院约翰逊堡海洋生物实验室，南卡罗来纳州查尔斯顿），尤金·D.特拉甘扎，巴里德·B.穆克吉，特拉维斯·B.格里芬，以及J.M.普雷斯科特。海洋细菌解蛋白气单胞菌新种的蛋白水解活性及一般特性。《细菌学杂志》87:1227 - 1233。1964年。——从海洋蛀木虫蛀木水虱的消化道中分离出一种高度蛋白水解的细菌物种。该生物体的形态和生化特性表明它是气单胞菌属的一个新物种，为此提出了“解蛋白气单胞菌”这一名称。刚分离出来时，该生物体生长需要海水；但是，在实验室长期培养后，只要提供相对大量的蛋白胨，它就能在盐浓度大大降低的培养基中生长。蛋白胨或酪蛋白水解物能够提供生长和蛋白酶产生所需的所有有机营养物。某些单个氨基酸也能够满足所有的能量、碳和氮需求。在柠檬酸盐存在的情况下能利用无机氮，但在葡萄糖存在时不能作为唯一的氮源。该生物体是兼性厌氧菌，但只有在剧烈通气的情况下才能实现最佳生长和蛋白酶产生。随着培养温度升高至最高40℃，24小时内获得的生长量迅速增加，但在42℃时不生长。
