MERKEL J R, BRAITHWAITE G D, KRITZLER H
J Bacteriol. 1964 Oct;88(4):974-80. doi: 10.1128/jb.88.4.974-980.1964.
Merkel, Joseph R. (College of Charleston, Charleston, S.C.), Gordon D. Braithwaite, and Henry Kritzler. Proteolytic attack of the chromoproteins of Porphyra by marine bacterial enzymes. J. Bacteriol. 88:974-980. 1964.-Three chromoproteins were isolated from the marine red alga, Porphyra leucosticta (Thuret), and were purified by adsorption chromatography on calcium phosphate gel and by ammonium sulfate fractionation. The algal chromoproteins were subjected to the proteolytic action of cultures of actively growing marine bacteria, marine bacterial culture filtrates, trypsin, and various marine bacterial enzyme preparations. Digestion of the chromoproteins was followed through various incubation intervals by measuring the loss of chromophore color and the increase in either ninhydrin-positive material (570 mmu) or 280-mmu light-absorbing material. There was good correlation between the loss of color and enzymatic hydrolysis of the proteins. this was particularly evident when a marine pseudomonad proteinase preparation was used as the proteolytic agent. In every digestion mixture, phycoerythrin was more resistant to degradation than was phycocyanin or allophycocyanin. Phycoerythrin was also more resistant to heat than was phycocyanin or allophycocyanin.
默克尔，约瑟夫·R.（查尔斯顿学院，南卡罗来纳州查尔斯顿），戈登·D. 布雷思韦特，以及亨利·克里茨勒。海洋细菌酶对紫菜色蛋白的蛋白水解攻击。《细菌学杂志》88:974 - 980。1964年。——从海洋红藻条斑紫菜（图雷）中分离出三种色蛋白，并通过磷酸钙凝胶吸附色谱法和硫酸铵分级分离法进行纯化。将藻类色蛋白置于活跃生长的海洋细菌培养物、海洋细菌培养滤液、胰蛋白酶以及各种海洋细菌酶制剂的蛋白水解作用下。在不同的孵育时间间隔内，通过测量发色团颜色的损失以及茚三酮阳性物质（570毫微米）或280毫微米吸光物质的增加来跟踪色蛋白的消化情况。颜色的损失与蛋白质的酶促水解之间存在良好的相关性。当使用海洋假单胞菌蛋白酶制剂作为蛋白水解剂时，这种相关性尤为明显。在每一种消化混合物中，藻红蛋白比藻蓝蛋白或别藻蓝蛋白更耐降解。藻红蛋白也比藻蓝蛋白或别藻蓝蛋白更耐热。
