用羊抗促黄体生成素释放激素γ球蛋白终止大鼠妊娠

Termination of pregnancy by sheep anti-LHRH gamma globulin in rats.

作者信息

Nishi N, Arimura A, de la Cruz K G, Schally A V

出版信息

Endocrinology. 1976 Apr;98(4):1024-30. doi: 10.1210/endo-98-4-1024.

Abstract

The effect of the administration of sheep anti-LHRH gamma-globulin (anti-LHRHG) on the maintenance of pregnancy was investigated in rats. Nidation was confirmed by laparotomy on day 7 or 8 of pregnancy. In one experiment, rats were then injected iv with 1 ml anti-LHRHG or normal sheep gamma-globulin (NSG) daily from days 7 to 11. The uteri were inspected again on day 14 of pregnancy, when it was found that complete resorption of the fetuses had occurred in the anti-LHRHG-treated rats, but that the fetuses were normal in the NSG-treated control rats. The effect of a single injection of 1 ml of anti-LHRHG on day 7, 8, 9, 10, 11, or 12 of pregnancy was also investigated. Administration on day 9 or 10 resulted in complete resorption of the fetuses by the time of the 2nd inspection on day 14, and treatment on day 8 or 11 was partially effective. However, treatment on day 7 or 12 exerted little effect on viability of the fetuses. None of the rats showed vaginal bleeding following treatment with anti-LHRHG. Termination of pregnancy by anti-LHRHG could be prevented by SC injection of 1 mug LHRH twice daily, or by 4 progesterone SC once daily, from days 9 through 12 of pregnancy. The ovaries of the rats treated with anti-LHRHG from days 7 to 11 were smaller than those of the NSG-treated control rats, and some of the corpora lutea underwent cystic degenerative changes. Lutein cells of the former were also smaller than those of the latter. Serum progesterone levels were reduced after a single injection of anti-LHRHG on day 9 or 10, but not on day 7 or 12 of pregnancy. There was excellent agreement between the reduction of serum progesterone and fetal resorption. Serum LH levels were low on days 7 through 12 in the anti-LHRHG-treated as well as the NSG-treated rats, and the possible suppressive effect of anti-LHRHG on LH could not be revealed because of insufficient sensitivity of the radioimmunoassay method. No significant difference was observed in serum prolactin levels between the groups of rats. The results clearly indicate that LHRH, by maintaining progesterone secretion, is indispensable on days 9 and 10 of pregnancy for the maintenance of pregnancy.

摘要

研究了给大鼠注射羊抗促黄体生成素释放激素γ球蛋白（抗LHRHG）对维持妊娠的影响。在妊娠第7天或第8天通过剖腹术确认着床情况。在一项实验中，从妊娠第7天至第11天，每天给大鼠静脉注射1毫升抗LHRHG或正常羊γ球蛋白（NSG）。在妊娠第14天再次检查子宫，发现抗LHRHG处理组的大鼠胎儿完全吸收，而NSG处理的对照组大鼠胎儿正常。还研究了在妊娠第7、8、9、10、11或12天单次注射1毫升抗LHRHG的效果。在第9天或第10天给药导致在第14天第二次检查时胎儿完全吸收，在第8天或第11天治疗部分有效。然而，在第7天或第12天治疗对胎儿活力影响很小。用抗LHRHG治疗后，没有大鼠出现阴道出血。从妊娠第9天至第12天，每天皮下注射1微克促黄体生成素释放激素两次，或每天皮下注射4次孕酮，可防止抗LHRHG终止妊娠。从第7天至第11天用抗LHRHG治疗的大鼠卵巢比NSG处理的对照组大鼠的卵巢小，一些黄体发生囊性退行性变化。前者的黄体细胞也比后者的小。在妊娠第9天或第10天单次注射抗LHRHG后血清孕酮水平降低，但在妊娠第7天或第12天未降低。血清孕酮降低与胎儿吸收之间有很好的一致性。抗LHRHG处理组和NSG处理组大鼠在第7天至第12天血清促黄体生成素水平都很低，由于放射免疫测定法灵敏度不足，无法揭示抗LHRHG对促黄体生成素可能的抑制作用。两组大鼠血清催乳素水平没有显著差异。结果清楚地表明，促黄体生成素释放激素通过维持孕酮分泌，在妊娠第9天和第10天对维持妊娠是必不可少的。