给大鼠注射抗促黄体激素释放激素的绵羊免疫球蛋白导致植入延迟。

Delayed implantation caused by administration of sheep immunogamma globulin against LHRH in the rat.

作者信息

Arimura A, Nishi N, Schally A V

出版信息

Proc Soc Exp Biol Med. 1976 May;152(1):71-5. doi: 10.3181/00379727-152-39331.

Abstract

The effect of the administration of sheep anti-LHRH gamma globulin (anti-LHRH-G) on implantation of fertilized ova was investigated in rats. Daily injections of 1 ml of anti-LHRH-G from Days 1 through 7 of pregnancy uniformly inhibited implantation of fertilized ova on Day 8, but viable sites, though considerably smaller in size than in control rats, became distinguishable on Day 14 in most rats. In some of these rats resorption of fetuses occurred, and others delivered pups 7-8 days after term. When the rats were given anti-LHRH-G from Days 3 through 5, the implantation was delayed by 5 days, but the gestation was not terminated. A single injection of 1 ml of anti-LHRH-G on Day 4 inhibited implantation on Day 8, but injection on Day 3 or 5 did not. The delayed implantation by anti-LHRH-G injected on Day 4 was nullified by concomitant administration of 2 sc injections of 1 mug of LHRH, or a single dose of 1 mug of estradiol. The data indicate that the hypothalamic LHRH is essentail on Day 4 of pregnancy for timely implantation of fertilized ova, probably by maintaining LH and, consequently, estrogen secretion.

摘要

在大鼠中研究了注射羊抗促黄体生成素释放激素γ球蛋白（抗LHRH-G）对受精卵着床的影响。从妊娠第1天至第7天每天注射1毫升抗LHRH-G，均能在第8天抑制受精卵着床，但在第14天，大多数大鼠中可辨认出存活部位，尽管其大小比对照大鼠中的明显小。在其中一些大鼠中发生了胎儿吸收，其他大鼠在足月后7-8天产仔。当大鼠在第3天至第5天给予抗LHRH-G时，着床延迟了5天，但妊娠未终止。在第4天单次注射1毫升抗LHRH-G可抑制第8天的着床，但在第3天或第5天注射则无此作用。在第4天注射抗LHRH-G所致的着床延迟可通过同时皮下注射2微克LHRH或单剂量1微克雌二醇而消除。数据表明，妊娠第4天，下丘脑LHRH可能通过维持LH分泌进而维持雌激素分泌，对受精卵及时着床至关重要。