Röyttä M, Frey H, Riekkinen P, Rinne U K
Eur Neurol. 1976;14(3):209-18. doi: 10.1159/000114742.
In the present study, six multiple sclerosis (MS) brain white matter biopsies were analyzed both morphologically and chemically. The purpose of this study was to test the reliability of the earlier postmortem observations. Special attention was given to proteins which were analyzed in polyacrylamide disc gel electrophoresis and to acid proteinase. The results show that myelin basic protein (BP) was present in all biopsies, although advanced demyelination was seen in one case as based on morphology. This finding is controversial to earlier observations. In addition to this, some acid proteins were decreased or lost in two cases. The activity of acid proteinases was increased from two- to fivefold in four of six cases. This increase of the activity was seen also in the normal-appearing MS autopsy white matter which served as control. The source of this activity is discussed. On the basis of the present results it is likely that the role of the BP in the breakdown of myelin in MS and the role of acid proteinases has to be studied in more detail.
在本研究中，对六份多发性硬化症（MS）脑白质活检样本进行了形态学和化学分析。本研究的目的是检验早期尸检观察结果的可靠性。特别关注了在聚丙烯酰胺圆盘凝胶电泳中分析的蛋白质以及酸性蛋白酶。结果显示，所有活检样本中均存在髓鞘碱性蛋白（BP），尽管根据形态学观察，其中一例出现了严重的脱髓鞘现象。这一发现与早期观察结果存在争议。除此之外，两例样本中的一些酸性蛋白质减少或缺失。六例中有四例酸性蛋白酶的活性提高了两到五倍。在作为对照的外观正常的MS尸检脑白质中也观察到了这种活性增加。本文讨论了这种活性的来源。基于目前的结果，很可能需要更详细地研究BP在MS髓鞘破坏中的作用以及酸性蛋白酶的作用。
