Miller A T, Hale D M, Alexander K D
J Cell Biol. 1965 Nov;27(2):305-12. doi: 10.1083/jcb.27.2.305.
When rat kidney slices were incubated in the presence of horseradish peroxidase, there was an energy-dependent uptake of the protein by the cells of the kidney tubules. The uptake was greatest in the proximal convoluted tubules and in the thick ascending limbs of the loops of Henle; it was abolished by cold, anoxia, 2,4-dinitrophenol, and fluoroacetate, and was more readily depressed by unfavorable metabolic conditions in the proximal convoluted tubules than in the thick ascending limbs. Protein uptake was inhibited when the kidney slices were incubated in electrolyte-free media. In sodium chloride solutions, uptake was reduced as sodium was progressively replaced by choline, and ouabain inhibited uptake in the proximal convoluted tubules, but not in the thick ascending limbs. To a limited extent, lithium could replace sodium in the incubation medium with no depression of peroxidase uptake. These results suggest that a sodium-stimulated, ouabain-sensitive ATPase may be involved in the uptake of protein by cells of the kidney tubule. The intracellular transport of peroxidase in cells of the proximal convoluted tubules was abolished by cold, anoxia, and 2,4-dinitrophenol, but it was not affected by concentrations of ouabain which inhibited the uptake of the protein.
当大鼠肾切片在辣根过氧化物酶存在的情况下进行孵育时，肾小管细胞对该蛋白质存在能量依赖性摄取。这种摄取在近端曲管和髓袢升支粗段最为显著；它会被低温、缺氧、2,4 -二硝基苯酚和氟乙酸所抑制，并且与髓袢升支粗段相比，近端曲管中不利的代谢条件更易使其摄取受到抑制。当肾切片在无电解质培养基中孵育时，蛋白质摄取受到抑制。在氯化钠溶液中，随着钠逐渐被胆碱取代，摄取量减少，哇巴因抑制近端曲管中的摄取，但不抑制髓袢升支粗段中的摄取。在一定程度上，锂可在孵育培养基中取代钠而不降低过氧化物酶的摄取。这些结果表明，一种钠刺激的、对哇巴因敏感的ATP酶可能参与肾小管细胞对蛋白质的摄取。近端曲管细胞中过氧化物酶的细胞内转运被低温、缺氧和2,4 -二硝基苯酚所阻断，但不受抑制蛋白质摄取的哇巴因浓度的影响。
