梨形四膜虫细胞核在整个细胞周期中的精细结构。
The fine structure of the nuclei of Tetrahymena pyriformis throughout the cell cycle.
作者信息
Flickinger C J
出版信息
J Cell Biol. 1965 Dec;27(3):519-29. doi: 10.1083/jcb.27.3.519.
Abstract
The fine structure of the nuclei of logarithmically growing Tetrahymena pyriformis, strain HSM, was studied at 30-minute intervals throughout the cell cycle. Organisms were selected at similar stages of cytokinesis by means of a braking pipette, incubated, fixed in OsO(4), and embedded in agar to facilitate subsequent preparation for electron microscopy. Aggregates of micronuclear chromatin underwent a decrease in density and number with a concomitant increase in size throughout interphase. There were no impressive changes in macronuclear morphology. It was found possible to estimate a cell's progress through interphase by observation of micronuclear morphology, but attempts to correlate changes in fine structure with periods of DNA synthesis were unsuccessful.
摘要
在整个细胞周期中，每隔30分钟研究对数生长期的梨形四膜虫HSM株细胞核的精细结构。通过制动移液管在胞质分裂的相似阶段选择生物体，进行孵育，用四氧化锇固定，并包埋在琼脂中，以利于后续的电子显微镜制备。在整个间期，微核染色质聚集体的密度和数量减少，同时大小增加。大核形态没有明显变化。发现通过观察微核形态可以估计细胞在间期的进程，但将精细结构的变化与DNA合成期相关联的尝试未成功。
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