烟草花叶病毒蛋白质的荧光研究

Fluorescent study of tobacco mosaic virus protein.

作者信息

Magne A, Gerard D, Hirth L, Laustriat G

出版信息

Biochim Biophys Acta. 1977 Dec 20;495(2):189-94. doi: 10.1016/0005-2795(77)90375-0.

Abstract

Protein fluorescence properties of tobacco mosaic virus [3 Trp residues per monomer (positions 17, 52, 152)] and of two tobacco mosaic virus mutants [green tomato atypical mosaic virus, 2 Trp (52, 152) and cucumber virus4, 1 Trp (unknown position)] have been studied. Emission spectra, fluorescence quantum yields and lifetimes were determined. Results showed that protein fluorescence is due to buried Trp only, except for the cucumber virus4 strain, in which Tyr also contributed to the emission. Comparison of the three strains showed that Trp 17 and Trp 52 have high fluorescence yields (phi17 = 0.29; phi52 = 0.37) whereas Trp 152 (probably present in cucumber virus4) is strongly quenched (phi152 = 0.035). An unusually efficient Tyr leads to Trp energy transfer was observed in tobacco mosaic virus protein, indicating that most of four Tyr residues are located near the highly fluorescent Trp.

摘要

对烟草花叶病毒[每个单体含3个色氨酸残基（位置17、52、152）]以及两种烟草花叶病毒突变体[绿番茄非典型花叶病毒，含2个色氨酸（52、152）和黄瓜病毒4，含1个色氨酸（位置未知）]的蛋白质荧光特性进行了研究。测定了发射光谱、荧光量子产率和寿命。结果表明，蛋白质荧光仅源于埋藏的色氨酸，但黄瓜病毒4毒株除外，在该毒株中酪氨酸也对发射有贡献。对这三个毒株的比较表明，色氨酸17和色氨酸52具有较高的荧光产率（φ17 = 0.29；φ52 = 0.37），而色氨酸152（可能存在于黄瓜病毒4中）被强烈淬灭（φ152 = 0.035）。在烟草花叶病毒蛋白中观察到一种异常高效的酪氨酸向色氨酸的能量转移，这表明四个酪氨酸残基中的大多数位于高荧光色氨酸附近。